Effects of Re-arterialization on early graft function and regeneration in the rat model of heterotopic auxiliary liver transplantation.
ABSTRACT In the rat model of heterotopic auxiliary liver transplantation, graft re-arterialization may influence the outcome of inter-liver competition. This was investigated in the current study using two transplanted groups with or without graft re-arterialization. Immediately after reperfusion, the re-arterialized grafts showed significantly higher bile flow rate and bilirubin excretion than the grafts without re-arterialization. DNA synthesis rate was also increased more drastically in the re-arterialized group following the transplantation. Without re-arterialization, the rats developed more pronounced cytolysis and cholestasis. Among the long-term survivors, all healthy re-arterialized grafts regenerated, whereas 5/6 non-re-arterialized grafts atrophied. These data demonstrate that the re-arterialization increases graft survival by improving early hepatic function, enhancing regenerative response and preventing post-transplant biliary complications in this rat model.
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ABSTRACT: Although auxiliary partial orthotopic liver transplantation (APOLT) has become a well-accepted procedure recently, a practical experiment model in APOLT using small animals has yet to be developed. Male Lewis rats were used for both donors and recipients. An auxiliary partial graft was obtained by ex vivo resection of the donor right and caudate lobes, and was transplanted orthotopically into the recipient after resection of the recipient medial and left hepatic lobes. Portal vein and hepatic duct reconstructions were by the cuff technique, and supra- and intrahepatic vena cava were sutured continuously. Operative outcomes, serum chemistry, liver tissue blood flow, angiographic and histopathological findings were then examined. Conventional orthotopic liver transplantation (OLT) procedures were also undertaken as a control. One-day, 1-week and 1-month survival rate of APOLT group was 100, 85 and 85%, respectively. AST in the APOLT group on the 1st postoperative day was significantly higher than in the OLT group. No significant differences were recognized in serum albumin and total bilirubin levels between the two groups. Although the portogram of an APOLT rat showed slight narrowing at the cuff anastomosis site, both the graft and the native liver were opacified similarly. The liver tissue blood flow on the 5th postoperative day in the native liver and the graft returned to as high as 95 and 74% of the values on laparotomy, respectively. Histological examinations of the auxiliary graft 1 month after transplantation showed mild ductular proliferation and mononuclear cell infiltration around the portal triads. This novel APOLT model in rats allows practical and reproducible results, and may be of value in the basic study of APOLT procedures.European Surgical Research 02/2000; 32(5):267-73. · 1.43 Impact Factor
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ABSTRACT: BACKGROUND: Rat liver transplantation is a common method in liver transplantation research. There are lots of different methods described until today. Although liver transplantation with retrograde reperfusion is established in the clinical routine there is no rat liver model described. METHODS: Arterialized rat liver transplantation with initial retrograde reperfusion was performed on n = 7 male LEWIS-(RT1)-rats. 1, 24 and 48 hours after the operation, serum parameters were determined. Furthermore, after 48 hours the liver was taken for histological assessment. RESULTS: The AST and the ALT levels showed a linear decrease during the first 48 hours after transplantation. GLDH levels showed an increase during the first 24 hours before they decreased strongly. In histology, the livers showed a good quality with only less necrosis. The highest amount of necrosis could be seen in the Rappaport zone 3. CONCLUSIONS: We were able to show that arterialized rat liver transplantation with initial retrograde reperfusion is feasible and shows a good outcome. Especially in centers where retrograde reperfusion of the liver is performed in clinical transplantation this method should be used for transplantation research to reach the closest possible relation between science and clinic.European Surgery 44(3). · 0.26 Impact Factor