Comparison of thermodynamic stabilities between PNA (peptide nucleic acid)/DNA hybrid duplexes and DNA/DNA duplexes

Department of Chemistry, Faculty of Science, Konan University, Kobe, Japan.
Nucleic Acids Symposium Series 02/1999; 42(42):93-4. DOI: 10.1093/nass/42.1.93
Source: PubMed


We have examined quantitatively stabilities of PNA/DNA hybrid duplexes with identical nearest-neighbor base pairs and compared stabilities between PNA/DNA and DNA/DNA. The average difference of stabilization energy of the short PNA/DNA was 0.9 kcal mol(-1), which suggests that the stability of the hybrids with identical nearest-neighbor base pairs can be predicted with the nearest-neighbor model as well as those of nucleic acid duplexes.

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    • "The physical properties of PNA endow them with specific advantages over standard oligonucleotides probes: they are less polar than (natural) nucleic acids and -as a consequence- PNA/DNA heteroduplexes are thermodynamically favoured when compared to the DNA/DNA double helix [8]. When very short PNA are used, this greater specificity allows the PNA/DNA heteroduplex to become thermodynamically unstable even when a single base-pair mismatch occurs [9]. Taking advantage of these unique PNA features we set-up a fluorescently-labelled PNA probe, coupled to FISH technology, to identify the presence of JAK2V617F at the single-cell level. "
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    07/2015; 3(1):15. DOI:10.1186/s40364-015-0039-y


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