Article

Identification of differentially expressed genes in nasopharyngeal carcinoma by means of the Atlas human cancer cDNA expression array.

Cancer Research Institute of Hunan Medical University, Changsha, PR China.
Journal of Cancer Research and Clinical Oncology (impact factor: 2.56). 08/2000; 126(7):400-6. DOI:10.1007/PL00008488 pp.400-6
Source: PubMed

ABSTRACT To investigate genes of critical areas, including cell cycle/growth control, apoptosis, oncogene/tumor suppressors and growth factor/cytokines, that are differentially expressed in nasopharyngeal carcinoma.
The Human Cancer cDNA Atlas, which contains 588 genes relating to tumor biology, was used to screen normal nasopharyngeal tissue, nasopharyngeal cancer (NPC). The reverse transcription/polymerase chain reaction was used to confirm the expression pattern of some genes identified by Atlas hybridization.
The differentially expressed cell cycle/growth control regulators in NPC showed a stronger tendency toward cell proliferation with the up-regulation of cyclin D1, cyclin D2 etc. The expression pattern of apoptosis-related genes demonstrated the up-regulation of both anti-apoptotic factors such as the BCL-2-related protein A1, TRAF3, the inhibitor of apoptosis protein A1 (IAPI) and apoptotic pathway elements such as Fas/Apo-1, Apo-2 ligand etc. Among oncogenes/tumor suppressors, MDM2, STAT1 and STAT2 were found to be up-regulated in NPC. The expression profile of growth factors/cytokines showed the up-regulation of many growth-enhancing factors such as EGR1, tumor-derived growth factor 1, platelet-derived growth factor A chain etc. as well as Th1-type cytokines e.g. interleukin-1beta and interferons. A smaller number of genes were down-regulated in nasopharyngeal cancer, such as those encoding ERK1, Raf, secreted apoptosis-related protein 1, CD27BP, transforming growth factor beta2, pre-B-cell-stimulating factor homologue etc.
The consistent tendency toward cell proliferation, the possibility of a stronger antiapoptotic force that operates on the normal apoptotic pathway, or the autocrine or paracrine growth factors may account for the development of NPC. Some genes are reported for the first time to have changed expression in nasopharyngeal carcinoma. The simple, quick, and high-throughput method of profiling gene expression by cDNA array hybridization provides us with a quick overview of key factors that may be involved in NPC, and may identify genes suitable for further study of carcinogenesis mechanism or targets for possible molecular diagnosis or therapy.

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Keywords

apoptosis protein A1
 
apoptosis-related genes
 
carcinogenesis mechanism
 
cell cycle/growth control
 
cell cycle/growth control regulators
 
consistent tendency
 
contains 588 genes
 
cyclin D1
 
encoding ERK1
 
genes suitable
 
growth factor beta2
 
Human Cancer cDNA Atlas
 
nasopharyngeal cancer
 
nasopharyngeal carcinoma
 
pre-B-cell-stimulating factor homologue
 
profiling gene expression
 
secreted apoptosis-related protein 1
 
stronger tendency
 
Th1-type cytokines
 
tumor-derived growth factor 1
 

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