Evaluation of some Moroccan medicinal plant extracts for larvicidal activity. J Ethnopharmacol 73(1-2):293-297

Laboratory of Medicinal Plants and Phytochemistry, Department of Biology, Faculty of Sciences - Semlalia, P.O. Box 2390 40000, Marrakech, Morocco.
Journal of Ethnopharmacology (Impact Factor: 3). 12/2000; 73(1-2):293-7. DOI: 10.1016/S0378-8741(00)00257-9
Source: PubMed

ABSTRACT The larvicidal properties of 16 extracts of four Moroccan medicinal plants: Calotropis procera (Wild.), Cotula cinerea (L.), Solanum sodomaeum (L.) and Solanum elaeagnifolium (CAV.) were tested against Anopheles labranchiae mosquito larvae. Among the extracts tested, nine exhibited high larvicidal activity with LC(50) (24 h) ranging from 28 to 325 ppm.

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    • "Recent studies have demonstrated that insecticidal components of chemicals derived from plants are active against specific target species, biodegradable, non toxic products and potentially suitable for use in integrated management programs (Markouk et al., 2000 and Tare et al., 2004). Nerium oleander L. (Apocynaceae) is an evergreen shrub widely used for ornamental purposes in the Mediterranean region (El- Shazly et al., 1996). "
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    ABSTRACT: Different concentrations (25, 30, 35 and 40%) of Nerium oleander L. leaf extracts were tested against the red grain beetle adults Tribolium castaneum (Herbst). Results showed that the percentage mortality ranged from 16.7% in the powder treatment increased to 70% in the alcohol extract at 40% concentration. Highest mortality rate was 49.2% in the alcohol extract, followed by 43.4% in the aqueous extract, while the least (30.9%) was recorded for the dry powder of N. oleander leaves. The results also showed an attraction and repellent effects of the extracts on the pest adults and there was a positive correlation between high mortality rate of the tested products and repellent effects on the adults, while a negative correlation was recorded between mortality rate and attractiveness.
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    • "Extractos de la planta en solventes orgánicos, como etanol y acetona, han presentado actividades insecticida (Moursy, 1997), larvicida (Markouk et al., 2000), antibacteriana y antiparasitaria (Larhsini et al., 1999). Una fracción de proteínas obtenida del extracto acuoso del tallo inhibió el crecimiento in vitro de Staphylococcus aureus y Enterobacter aerogenes a una concentración de 25 µg/mL con una eficiencia comparable a la del cloramfenicol utilizado como control positivo (Samy y Chow, 2012). "
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    ABSTRACT: y del Caribe de Plantas Medicinales y Aromáticas 12 (5): 476 -492 ISSN 0717 7917 Artículo Original | Original Article 476 Citotoxicidad in vitro de extractos laticiferos de Calotropis procera (Aiton) W.T. Aiton y Pedilanthus tithymaloides (L.) Poit. [In vitro cytotoxicity of laticifers extracts from Calotropis procera (Aiton) W.T and Pedilanthus tithymaloides (L.) Poit.] Abstract In order to assess the anticancer action of extracts obtained by latex from Calotropis procera and Pedilanthus tithymaloides, samples were collected from adult plants. Soluble proteins were extracted with 16 µL of 50 mM sodium acetate pH 5/μg integral latex and centrifugation at 16,000 x g for 15 min, the supernatant was named "latex crude extract" (LCE). The "latex methanolic extract" (LME) was obtained on dried latex. Both extracts were tested in vitro by cytotoxic and cytostatic activity in Jurkat cell cultures. Cellular viability, proliferation, necrosis and apoptosis were evaluated. LCE and LME of C. procera were found with cytotoxic and cytostatic activity after 24 incubation hours (p < 0,05) with doses from 1µg/mL. The LCE and LME of P. tithymaloides presented cytotoxic effect (p < 0,05) from 50 µg/mL and from 1µg/mL, respectively. Resumen Con el objetivo de evaluar el potencial anticanceroso de extractos de látex de Calotropis procera y Pedilanthus tithymaloides se colectaron muestras de plantas adultas. Las proteínas solubles fueron extraídas con 16 µL de acetato de sodio 50 mM pH 5/µg de látex integral y centrifugación a 16.000 x g durante 15 min, denominándose al sobrenadante "extracto crudo de látex" (ECL). El "extracto metanólico de látex" (EML) se obtuvo sobre látex deshidratado. Ambos extractos fueron probados en su actividad citotóxica y citostática in vitro sobre cultivos de células Jurkat. Se realizaron estudios de viabilidad, proliferación, necrosis y apoptosis celular. El ECL y el EML de C. procera presentaron actividad citotóxica y citostática después de 24 y 48 horas de incubación (p < 0,05) con dosis desde 1 µg/mL. Los ECL y EML de P. tithymaloides presentaron efectos citotóxicos (p < 0,05) a partir de 50 µg/mL y desde 1 µg/mL respectivamente. Palabras Clave: Látex, cultivos celulares in vitro, células Jurkat, citotóxico, citostático.
    Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas 09/2013; 12(5):476-492. DOI:10.13140/2.1.3567.6802 · 0.33 Impact Factor
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    • "As a result, research on alternative control methods, such as microbial and botanical insecticides, has been carried out over the last few years. Botanical insecticides are environmentally friendly , readily available, and relatively safe for non-target pests (Markouk et al. 2000; Isman 2006; Jbilou et al. 2008; Pavela 2008; Shekari et al. 2008; Cheng et al. 2009; Govindarajan et al. 2012a). To date, no studies have shown resistance to botanical-based insecticides among vector pests due to the limited use of botanical agents in vector control programs (Shaalan et al. 2005). "
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    ABSTRACT: This study focuses on the larvicidal, oviposition, and ovicidal effects of a crude extract of Artemisia annua against Aedes aegypti, Anopheles sinensis, and Culex quinquefasciatus. Dried cells of Artemisia annua from cell suspension cultures were extracted using hexane. The extract showed moderate larvicidal effects against mosquitoes. At 24-h post treatment, the LC50 values for Anopheles sinensis, Aedes aegypti, and Culex quinquefasciatus were recorded as 244.55, 276.14, and 374.99 ppm, respectively. The percentage mortality of larvae was directly proportional to the tested concentration. Anopheles sinensis was found to be the most susceptible species, whereas Culex quinquefasciatus was the most tolerant to the Artemisia annua extract. The results indicated that the Artemisia annua extract showed concentration-dependent oviposition deterrent activity and had a strong deterrent effect. At 500 ppm, the percentage effective repellency was more than 85 % compared with the control group for all the species, with oviposition activity index values of -0.94, -0.95, and -0.78 for Aedes aegypti, Anopheles sinensis, and Culex quinquefasciatus, respectively. In the ovicidal assay, the percentage hatchability of eggs after treatment with 500 ppm of Artemisia annua extract was significantly lower than the control, with values of 48.84 ± 4.08, 38.42 ± 3.67, and 79.35 ± 2.09 % for Aedes aegypti, Anopheles sinensis, and Culex quinquefasciatus, respectively. Artemisia annua was found to be more effective against Aedes aegypti and Anopheles sinensis compared with Culex quinquefasciatus. This study indicated that crude extract of A. annua could be a potential alternative for use in vector management programs.
    Parasitology Research 07/2013; 112(9). DOI:10.1007/s00436-013-3506-0 · 2.10 Impact Factor
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