The specific expression of three novel splice variant forms of human metalloprotease-like disintegrin-like cysteine-rich protein 2 gene inBrain tissues and gliomas

Department Biochemistry, Graduate School of Medical Sciences, Kyushu University, Higashi-ku, Fukuoka 812-8582, Japan.
Japanese journal of cancer research: Gann 11/2000; 91(10):1001-6.
Source: PubMed


We have previously identified 67 exons on a yeast artificial chromosome contig spanning 1.5 Mb around the multidrug resistance 1 gene region of human chromosome 7q21.1. In this study, we identified three novel cytoplasmic variants (MDC2-gamma, MDC2-delta, and MDC2-epsilon) of the human metalloprotease-like disintegrin-like cysteine-rich protein 2 (MDC2) among these exons by screening a human brain cDNA library and also by using a reverse transcription polymerase chain reaction. Genomic sequence analysis strongly supported the idea that the variations in the cytoplasmic domain were generated by alternative splicing. The expression of MDC2 variant forms in human brain tissue and gliomas was examined by reverse transcription polymerase chain reaction and RNase protection assay. MDC2-epsilon was expressed only in the cortical and hippocampal regions in human brain, but not in gliomas. In contrast, MDC2-gamma was a major form expressed in human gliomas. Specific expression of these cytoplasmic variants of MDC2 in human brain and its malignancies is discussed.

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    • "Yagami-Hiromasa et al. 1995; 9 Harris et al. 1997; 10 Gilpin et al. 1998; 11 Kratzschmar et al. 1996; 12 Patel et al. 1998; 13 Frayne et al. 2002; 14 Kurisaki et al. 1998; 15 Poindexter et al. 1999; 16 Harada et al. 2000; 17 Cal et al. 2000; 18 Roberts et al. 1999; 19 Howard et al. 2000; 20 Howard et al. 2001; 21 Cerretti et al. 1999; 22 Xu et al. 1999; 23 Yoshinaka et al. 2002 MP active refers to either predicted (p) or already demonstrated (d) activity based on the amino acid sequence of the catalytic active site in metalloprotease domain ADAM12 is a member of ADAMs family, which has been implicated in cell adhesion, cell fusion and fusion signalling (Wolfsberg et al. 1995). ADAM12 comprises a pro-domain, a metalloprotease domain, a disintegrin domain, a cystein-rich domain, an epidermal growth factor (EGF)-like domain, and a transmembrane domain with an attached cytoplasmic tail. "
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