Immunological methods for the diagnosis of bovine tuberculosis - State of the art
Bundesinstitut für gesundheitlichen Verbraucherschutz und Veterinärmedizin, Jena.Berliner und Münchener tierärztliche Wochenschrift (Impact Factor: 0.82). 11/2000; 113(10):388-91.
An overview is given about the most common immunological methods for the diagnosis of bovine tuberculosis. These methods are based on cell mediated immune reactions on the one hand (intradermal skin test, interferon-gamma-test, lymphocyte transformation test) or on antibody production against Mycobacterium bovis on the other hand (ELISA). The advantages and disadvantages of the methods as well as their specificity and sensitivity are discussed.
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ABSTRACT: Introduction: The isolation and identification of mycobacteria causing tuberculosis in man and domestic animals are difficult and time consuming. Therefore, methods for a faster and more accurate bacteriological detection are required. For this purpose, we evaluated a polymerase chain reaction for the detection of Mycobacterium avium. Material and methods: A PCR method based on the repetitive IS1245 sequence was evaluated for the detection of Mycobacterium avium in 154 clinical specimens, collected during the years 1998 and 1999. The material was treated with N-acetyl-L-cystein-NaOH. 700 μl of treated material was frozen at -20°C for further DNA isolation and the rest was inoculated on the egg media with pyruvate (IUAT-P) or glycerol (IUAT-G), Stonebrink, Middlebrook 7H10 and fluid media MGIT. Results: By the microscopical examination of Ziehl-Neelsen stained smears, 28 (18.2 %) samples were found positive for acidfast bacteria. From 81 (52.6 %) specimens, mycobacteria were isolated and 69 isolates were identified as M. avium species. A PCR based on the IS1245 sequence was used to detect M. avium in tissues. Using 145 specimens, the diagnostic sensitivity, diagnostic specificity, positive and negative predictive values were found to be 91.3 %, 92.1 %, 91.3 % and 92.1 %, respectively. Conclusion: We conclude that the IS1245-specific PCR described herein represents an important additional tool in the diagnosis of mycobacteria. By considering both the PCR results together with the anamnestic data, the sensitivity of a classical bacteriological investigation can be increased.Wiener Tierarztliche Monatsschrift 01/2004; 91(10):254-257.
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