Evidence of Bartonella henselae infection in cats and dogs in the United Kingdom

Department of Veterinary Clinical Science and Animal Husbandry, University of Liverpool.
The Veterinary record (Impact Factor: 1.49). 01/2001; 147(24):673-7. DOI: 10.1136/vr.147.24.673
Source: PubMed


Sera from cats and dogs in the UK were tested by ELISA for antibodies to Bartonella henselae. Seropositivity was confirmed in 28 of 69 pet cats (40.6 per cent), 33 of 79 feral cats (41.8 per cent) and three of 100 pet dogs. Reactivity to specific B. henselae antigens was confirmed by Western blotting and demonstrated that consistent antigenic bands were bound by sera from the cats and dogs.

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Available from: Stuart D Carter, Jun 06, 2014
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    • "In Denmark , Chomel et al. (2002) detected B. henselae antibodies in 46.7% of the cats studied. The B. henselae seroprevalence reported in cats was similar in Denmark and other European countries (Barnes et al., 2000; Gurfield et al., 2001). Lower percentages of B. henselae seroprevalence in cats were reported in Switzerland (8.3%) and in Germany (15%) (Glaus et al., 1997; Haimerl et al., 1999). "
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    ABSTRACT: Bartonella henselae is considered an emerging pathogen of veterinary and medical interest that can be occasionally transmitted to humans. Cats are considered to be the only reservoir host for B. henselae. In this study, we used a nested-PCR assay to investigate the prevalence of B.henselae and Bartonella clarridgeiae DNA in peripheral blood samples, fine needle lymph node aspirate specimens and oral swabs from 85 cats in order to develop an easy diagnostic strategy for the selection of infection-free cats that are being considered as pets, especially for immunocompromised patients. Overall, molecular analysis showed that 71 cats (83.5%) tested PCR positive for the presence of B. henselae DNA. PCR amplification of DNA B. henselae produced positive products from lymph node aspirate specimens (62/85; 72.9%) similar to those obtained from blood samples (60/85; 70.6%) and higher than those from oral swabs (51/85; 60%) of cats. No PCR product was obtained for B. clarridgeiae. The simultaneous analysis of three different clinical samples in our study increased the diagnostic possibilities for B. henselae infection in the examined cats from 60-72.9% to 83.5%. Lymph node aspirates were found to be the most effective clinical samples for the detection of B. henselae and blood samples were the next best. Oral swab samples were used in this study with good results when considered in combination with blood and/or lymph node aspiration. The use of nested-PCR assay on these three clinical samples may enhance the diagnostic sensitivity for bartonellosis in cats irrespective of the clinical status of animals.
    Research in Veterinary Science 12/2009; 88(3):379-84. DOI:10.1016/j.rvsc.2009.11.005 · 1.41 Impact Factor
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    • "Recently, B. henselae DNA has been amplified and sequenced from the liver of a dog with peliosis hepatitis [37] and a dog with granulomatous hepatitis [24] and from the blood of three dogs with either fever, thrombocytopenia or neurologic dysfunction [51]. Three canine serosurveys carried out in Hawaii, Japan and the United Kingdom describe B. henselae seroprevalence of 6.5% [20], 7.7% [61] and 3% [4], respectively. In Japan, B. henselae PCR positive results were also reported from peripheral blood, nail clippings and oral swabs in 15% of the dogs studied [61]. "
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    ABSTRACT: In contrast to the large body of literature regarding Bartonella henselae in humans and cats, there is little information about B. henselae as an infectious agent in dogs. Due to the paucity of information regarding the B. henselae serology in dogs, we performed a cross-sectional serosurvey using B. henselae antigen in order to compare the seroprevalence between sick and healthy dogs from the south-eastern USA. Ninety-nine sera were collected from clinically healthy dogs. Three hundred and one sera from sick dogs were submitted to North Carolina State University for serologic screening against a panel of arthropod-transmitted organisms. Serological tests were performed using B. henselae (Bh), Rickettsia rickettsii (Rr), Ehrlichia canis (Ec), Bartonella vinsonii subspecies berkhoffii (Bvb), Babesia canis (Bc) and Borrelia burgdorferi (Bb) antigens. Serum B. henselae IgG antibodies were detected in 10.1% of healthy dogs and in 27.2% of sick dogs. The difference in seroprevalence between the two groups was statistically significant. The majority of seroreactive dogs (80%) had low titers of 1:64 or 1:128. In healthy dogs, seroprevalence for Rr was 14.1% and for Bvb was 1%. In sick dogs, Rr seroprevalence was 29.7%, Ec 6.5%, Bvb 4.7%, Bb 1.7% and Bc was 0.85%. Of the sick dogs that were seroreactive to B. henselae antigens, 40.6% were also seroreactive to Rr, 15.0% reactive to Bvb antigens, 14.8% reactive to Ec antigens, 1.8% reactive to Bc antigens and 1.75% reactive to Bb antigens. Sera from dogs experimentally infected with B. vinsonii subsp. berkhoffii, E. canis or R. rickettsii did not cross react with B. henselae antigens, by IFA testing. This study indicates that B. henselae IgG antibodies are prevalent in healthy and sick dogs living in the south-eastern USA. Nevertheless, further studies are needed to evaluate the epidemiological, clinical and zoonotic relevance of B. henselae infection in dogs.
    Veterinary Research 09/2004; 35(5):585-95. DOI:10.1051/vetres:2004034 · 2.82 Impact Factor
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    ABSTRACT: Fifty dogs fromthe area around Barcelona, Spain, were evaluated for serologic evi- dence of exposure to vector-borne pathogens. Dirofilaria immitis, E h r l i c h i a c a n i s, Borrelia burgdorferi, L e i s h m a n i a infantum, Bartonella vinsonii s u b s p e c i e s b e r k h o f f i i ,and Rickettsia rickettsiia n t i g e n s were used for testing purposes. Seroreactivity was determined in 3 different groups of dogs that were categorized based upon their L infantum infection status: uninfected healthy dogs (group 1), L infan- tum-infected healthy dogs (group 2), and L infantum-infected dogs with clinical mani- festations consistent with leishmaniasis (group 3).Of the 50 dogs included in this study, 49 had serologic evidence of expo- sure to at least 1 organism for which testing
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