Article

Microfabricated reaction and separation systems.

Department of Chemical Engineering, University of Michigan, 2300 Hayward, 3022 HH Dow, Ann Arbor, MI 48109-2136, USA.
Current Opinion in Biotechnology (Impact Factor: 8.04). 03/2001; 12(1):92-8. DOI: 10.1016/S0958-1669(00)00166-X
Source: PubMed

ABSTRACT Over the past year there have been a number of recent advances in the fields of miniaturized reaction and separation systems, including the construction of fully integrated 'lab-on-a-chip' systems. Microreactors, which initially targeted DNA-based reactions such as the polymerase chain reaction, are now used in several other chemical and biochemical assays. Miniaturized separation columns are currently employed for analyzing a wide variety of samples including DNA, RNA, proteins and cells. Although significant advances have been made at the component level, the realization of an integrated analysis system still remains at the early stages of development.

1 Follower
 · 
70 Views
  • [Show abstract] [Hide abstract]
    ABSTRACT: Because of strong demands for high throughput or high content cell-based assay, significant efforts have focused on the assay miniaturization by fabricating cell microarray using a variety of cell patterning techniques such as spotting, photolithography or soft lithography and by integrating cell microarray into microfluidic devices. Response of cells cultured on microarray can be monitored by using either electrochemical or optical detection methods. Impedancebased detection and potential-based detection have been widely used for electrochemical detection, while optical detection relies mostly on the fluorescence and bioluminescence-based techniques. Resultant cell microarray-based biosensor can be applied for high throughput/content drug screening and detection of pathogens, pollutants and warfare agents. For the successful application of cell-based biosensors to various areas, multi-phenotypic cell microarray should be developed and cells on microarray must be cultured in 3-dimensional environment as they do in real tissue to obtain accurate response of cells against target analytes.
    09/2013; 3(3):117-130. DOI:10.1007/s13534-013-0103-1
  • [Show abstract] [Hide abstract]
    ABSTRACT: In this study, a micro-pump for Bio-MEMS by using a new bio-compatible piezoelectric thin film is newly developed, which could be built in DDS and HMS. At first, we carried out the performance assessment of our piezoelectric thin film pump of a newly designed micro-fluid system by using the finite element method, which can consider the interaction between the piezoelectric solid and the fluid. The results of numerical analyses show a enough transportation ability of our micro-pump system. Then, we generate multilayer MgSiO3 thin film on Cu/Ti/Si(100) substrate by using RFmagnetron sputtering method. We measured the crystallographic orientation and piezoelectric property and confirmed that MgSiO3(101) crystal has grown well. The strain constant d33 was calculated by using the displacement-voltage curve, such as 179.4pm/V. Further, the deflection and frequency of the monomorph-actuator, which fabricated by using the micro-machining process, were measured by the laser doppler vibrometer. It showed that the deflection linearly increased with applied voltage, and it was 82.6nm with the applied voltage of 15V. We evaluated the flow rate of micropump using the luminance difference measurement method. The results showed that the maximum flow rate was 7.1nl/s at the applied voltage of 15V. It shows the possibility of Bio-MEMS device by using our newly developed micro-pump with a new bio-compatible piezoelectric material MgSiO3.
    Proceedings of SPIE - The International Society for Optical Engineering 03/2011; DOI:10.1117/12.881760 · 0.20 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Random cationic copolymer brushes composed of 2-(dimethylamino)ethyl methacrylate (DMAEMA) and N-isopropylacrylamide (NIPAAm) were synthesized using the atom transfer radical polymerization (ATRP) method. The effects of varying the monomer feed ratios (30:70 and 70:30 DMAEMA:NIPAAm) and polymerization times on the film height, morphology and stimuli response to pH of the brush were evaluated. While the polymerization time was found to have little influence on the properties of the brushes, the monomer feed ratios had a great impact. The 70 % DMAEMA polymer brush had similar height as the 30 % DMAEMA brush after 45 min; however, it had a greater response to pH and morphological change compared to the 30 % DMAEMA. The 70 % DMAEMA brush was used to demonstrate an efficient approach to alleviate the ion suppression effect in MALDI analysis of complex mixtures by effectively fractionating a binary mixture of peptides prior to MALDI-MS analysis.
    Polymer 08/2014; 55(16). DOI:10.1016/j.polymer.2014.06.075 · 3.77 Impact Factor