Protective Cytotoxic T Lymphocyte Responses Induced by DNA Immunization against Immunodominant and Subdominant Epitopes of Listeria monocytogenes Are Noncompetitive

Second Department of Internal Medicine, Hamamatsu Univeristy School of Medicine, Hamamatsu 431-3192, Japan.
Infection and Immunity (Impact Factor: 3.73). 06/2001; 69(5):3427-30. DOI: 10.1128/IAI.69.5.3427-3430.2001
Source: PubMed


Taking advantage of the fact that plasmid DNA encoding a single cytotoxic T lymphocyte (CTL) epitope can induce CTLs, we examined
the influence of T-cell responses to dominant epitopes on those to a subdominant epitope derived from Listeria monocytogenes. Our data suggest that interaction between T cells against dominant and subdominant epitopes does not operate in the generation
of the hierarchy. Furthermore, we found that a single dominant epitope is sufficient for the induction of protective immunity.

Download full-text


Available from: Yukio Koide, Mar 11, 2014
  • Source
    • "They administered in mice with the plasmid by intramuscular injection or gene gun-mediated intradermal injection and showed that the DNA vaccination successfully induced each epitope-specific CTL activity. Results of our single CTL-epitope DNA vaccine showed that a single dominant CTL epitope is sufficient for the induction of protective immunity [39], suggesting that selecting the most effective CTL epitope for each pathogen is critical for the efficacy of DNA vaccines. "
    [Show abstract] [Hide abstract]
    ABSTRACT: For protection against intracellular bacteria such as Mycobacterium tuberculosis and Listeria monocytogenes, the cellular arm of adaptive immunity is necessary. A variety of immunization methods have been evaluated and are reported to induce specific CD8(+) T cells against intracellular bacterial infection. Modified BCG vaccines have been examined to enhance CD8(+) T-cell responses. Naked DNA vaccination is a promising strategy to induce CD8(+) T cells. In addition to this strategy, live attenuated intracellular bacteria such as Shigella, Salmonella, and Listeria have been utilized as carriers of DNA vaccines in animal models. Vaccination with dendritic cells pulsed with antigenic peptides or the cells introduced antigen genes by virus vectors such as retroviruses is also a powerful strategy. Furthermore, vaccination with recombinant lentivirus has been attempted to induce specific CD8(+) T cells. Combinations of these strategies (prime-boost immunization) have been studied for the efficient induction of intracellular bacteria-specific CD8(+) T cells.
    BioMed Research International 05/2010; 2010:764542. DOI:10.1155/2010/764542 · 2.71 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Listeria epitope-specific helper T (Th) cells were able to be primed and induced in vivo by immunization with a plasmid carrying an invariant chain (Ii) gene whose class II-associated invariant chain peptide (CLIP) region was replaced by a Listeria Th epitope. Immunization of C3H/He mice with an Ii-LLO 215-226 plasmid induced specific interferon-gamma- and interleukin 2-producing Th cells and conferred significant protective immunity against listerial infection.
    Infection and Immunity 06/2002; 70(5):2676-80. DOI:10.1128/IAI.70.5.2676-2680.2002 · 3.73 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The complete genome sequences of more than 60 microbes have been completed in the past decade. Concurrently, a series of new informatics tools, designed to harness this new wealth of information, have been developed. Some of these new tools allow researchers to select regions of microbial genomes that trigger immune responses. These regions, termed epitopes, are ideal components of vaccines. When the new tools are used to search for epitopes, this search is usually coupled with in vitro screening methods; an approach that has been termed computational immunology or immuno-informatics. Researchers are now implementing these combined methods to scan genomic sequences for vaccine components. They are thereby expanding the number of different proteins that can be screened for vaccine development, while narrowing this search to those regions of the proteins that are extremely likely to induce an immune response. As the tools improve, it may soon be feasible to skip over many of the in vitro screening steps, moving directly from genome sequence to vaccine design. The present article reviews the work of several groups engaged in the development of immuno-informatics tools and illustrates the application of these tools to the process of vaccine discovery.
    Immunology and Cell Biology 07/2002; 80(3):255-69. DOI:10.1046/j.1440-1711.2002.01092.x · 4.15 Impact Factor
Show more