Article

Effects of different carbohydrate sources on the growth of Tuber borchii Vittad. mycelium strains in pure culture.

Istituto di Chimica Biologica Giorgio Fornaini, Università degli Studi di Urbino, PU, Italy.
Molecular and Cellular Biochemistry (impact factor: 2.06). 03/2001; 218(1-2):65-70. pp.65-70
Source: PubMed

ABSTRACT The influence of carbohydrate utilisation on the growth of three strains of Tuber borchii Vittad. mycelium (1BO, 17BO and 10RA) in culture was assessed using culture media containing glucose (control), mannose or mannitol. Mannose was the best substrate for growth of the strains and this was particularly evident for strain 17BO. Mannitol instead was metabolized only by 10RA and 1BO. In order to explain the different growth trends, analyses of enzyme levels, kinetic parameters, protein patterns and the morphology of the three strains were carried out. Our results show that these strains of T. borchii mycelium were affected by the substrates used in the media. The aim of the present work was to optimise the in vitro production of T. borchii mycelium for use in experiments which require the fungus in precise and reproducible conditions, such as mycorrhizal synthesis or protein and nucleic acid extractions.

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Keywords

carbohydrate utilisation
 
different growth trends
 
fungus
 
kinetic parameters
 
Mannitol
 
Mannose
 
mycelium
 
mycorrhizal synthesis
 
nucleic acid extractions
 
present work
 
protein patterns
 
reproducible conditions
 
strain 17BO
 
strains
 
T. borchii mycelium
 
three strains
 
Tuber borchii Vittad
 
vitro production