Therapeutic potential of curcumin in human prostate cancer. III. Curcumin inhibits proliferation, induces apoptosis, and inhibits angiogenesis of LNCaP prostate cancer cells in vivo
ABSTRACT Earlier work from our laboratory highlighted the therapeutic potential of curcumin (turmeric), used as a dietary ingredient and as a natural anti-inflammatory agent in India and other Southeast Asian countries. This agent was shown to decrease the proliferative potential and induce the apoptosis potential of both androgen-dependent and androgen-independent prostate cancer cells in vitro, largely by modulating the apoptosis suppressor proteins and by interfering with the growth factor receptor signaling pathways as exemplified by the EGF-receptor. To extend these observations made in vitro and to study the efficacy of this potential anti-cancer agent in vivo, the growth of LNCaP cells as heterotopically implanted tumors in nude mice was followed.
The androgen-dependent LNCaP prostate cancer cells were grown, mixed with Matrigel and injected subcutaneously into nude mice. Experimental group received a synthetic diet containing 2% curcumin for up to 6 weeks. At the end point, sections taken from the excised tumors were evaluated for pathology, cell proliferation, apoptosis, and vascularity.
Curcumin causes a marked decrease in the extent of cell proliferation as measured by the BrdU incorporation assay and a significant increase in the extent of apoptosis as measured by an in situ cell death assay. Moreover, a significant decrease in the microvessel density as measured by the CD31 antigen staining was also seen.
Curcumin could be a potentially therapeutic anti-cancer agent, as it significantly inhibits prostate cancer growth, as exemplified by LNCaP in vivo, and has the potential to prevent the progression of this cancer to its hormone refractory state.
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ABSTRACT: Curcuma longa Linnaeus and Zingiber officinale Roscoe are two main representatives of Zingiberaceae family studied for a wide range of therapeutic properties, including: antioxidant, anti-inflammatory, anti-angiogenic, antibacterial, analgesic, immunomodulatory, proapoptotic, anti-human immunodeficiency virus properties and anticancer effects. This study was aimed to analyse the ethanolic extracts of Curcuma rhizome (Curcuma longa Linnaeus) and Zingiber rhizome (Zingiber officinale Roscoe) in terms of polyphenols, antioxidant activity and anti-melanoma potential employing the B164A5 murine melanoma cell line. In order to evaluate the total content of polyphenols we used Folin-Ciocâlteu method. The antioxidant activity of the two ethanolic extracts was determined by DPPH assay, and for the control of antiproliferative effect it was used MTT proliferation assay, DAPI staining and Annexin-FITC-7AAD double staining test. Results showed increased polyphenols amount and antioxidant activity for Curcuma rhizome ethanolic extract. Moreover, 100 μg/ml of ethanolic plant extract from both vegetal products presented in a different manner an antiproliferative, respectively a proapoptotic effect on the selected cell line. The study concludes that Curcuma rhizome may be a promising natural source for active compounds against malignant melanoma.Biological research 01/2015; 48(1):1. DOI:10.1186/0717-6287-48-1 · 1.04 Impact Factor
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ABSTRACT: Numerous treatment modalities are available for variety of systemic and dental diseases; however, the main drawback of conventional medicinal therapies is its various side effects causing harm to the patient. This diverted the interest of researchers towards an alternative approach where natural compounds derived from plants could be used for treating those patients. Curcumin is such an alternative which exhibits a number of medicinal properties and has been used from the centuries. This article discusses the efficacy of curcumin in maintenance of oral health, in particular, and overall health, in general. Curcumin (diferuloylmethane) is a polyphenol derived from the curcuma longa plant, commonly known as turmeric, is a herb known for its medicinal properties. It has a variety of therapeutic properties like anti-inflammatory, anti-oxidant, anti-microbial, hepatoprotective, immunostimulant, antiseptic , anti-angiogenic, apoptotic and anti-mutagenic. All these beneficial properties makes this compound quite more useful in dental field especially in treating periodontal diseases and cancers involving head and neck region and oral cavity. It can also be formulated as a pit and fissure sealant, mouth wash, and subgingival irrigant in different preparations in different dosages and also as a component in local drug delivery system in gel form. INTRODUCTION Most of the anti-cancer drugs are very toxic, highly inefficient for cancer treatment or highly expensive. Thus, these drugs show their limited potential in cancer therapy and beyond the reach of the majority of affected individuals. Hence, an alternative medicinal product without exhibiting such drawbacks will be good option as an anti-cancer drug. Natural phytochemicals isolated from plants used as traditional medicines are referred as such sources. Curcumin, derived from the dried rhizome of the East Indian turmeric plant (Curcuma longa), a perennial herb belonging to the Zingiberacae (ginger) family of botanicals, has drawn attention as alternative source in cancer therapy. This turmeric plant is 3 feet in height and Corresponding AuthorInternational Journal of Biological & Pharmaceutical Research 01/2015; 2015(64):281-290.
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ABSTRACT: Cancer is the most dreadful disease worldwide in terms of morbidity and mortality. The exact cause of cancer development and progression is not fully known. But it is thought that cancer occurs due to the structural and functional changes in the genes. The current approach to cancer treatment based on allopathic is expensive, exhibits side effects; and may also alter the normal functioning of genes. Thus, a safe and effective mode of treatment is needed to control the cancer development and progression. Some medicinal plants provide a safe, effective and affordable remedy to control the progression of malignant cells. The importance of medicinal plants and their constituents has been documented in Ayurveda, Unani medicine, and various religious books. Curcumin, a vital constituent of the spice turmeric, is an alternative approach in the prevention of cancer. Earlier studies have shown the effect of curcumin as an antioxidant, antibacterial, antitumor and it also has a noteworthy role in the control of different diseases. In this review, we summarize the understanding of chemopreventive effects of curcumin in the prevention of cancer via the regulation of various cell signaling and genetic pathways.BioMed Research International 09/2014; 2014:761608. DOI:10.1155/2014/761608 · 2.71 Impact Factor