Therapeutic potential of curcumin in human prostate cancer. III. Curcumin inhibits proliferation, induces apoptosis, and inhibits angiogenesis of LNCaP prostate cancer cells in vivo
ABSTRACT Earlier work from our laboratory highlighted the therapeutic potential of curcumin (turmeric), used as a dietary ingredient and as a natural anti-inflammatory agent in India and other Southeast Asian countries. This agent was shown to decrease the proliferative potential and induce the apoptosis potential of both androgen-dependent and androgen-independent prostate cancer cells in vitro, largely by modulating the apoptosis suppressor proteins and by interfering with the growth factor receptor signaling pathways as exemplified by the EGF-receptor. To extend these observations made in vitro and to study the efficacy of this potential anti-cancer agent in vivo, the growth of LNCaP cells as heterotopically implanted tumors in nude mice was followed.
The androgen-dependent LNCaP prostate cancer cells were grown, mixed with Matrigel and injected subcutaneously into nude mice. Experimental group received a synthetic diet containing 2% curcumin for up to 6 weeks. At the end point, sections taken from the excised tumors were evaluated for pathology, cell proliferation, apoptosis, and vascularity.
Curcumin causes a marked decrease in the extent of cell proliferation as measured by the BrdU incorporation assay and a significant increase in the extent of apoptosis as measured by an in situ cell death assay. Moreover, a significant decrease in the microvessel density as measured by the CD31 antigen staining was also seen.
Curcumin could be a potentially therapeutic anti-cancer agent, as it significantly inhibits prostate cancer growth, as exemplified by LNCaP in vivo, and has the potential to prevent the progression of this cancer to its hormone refractory state.
- SourceAvailable from: Suryakant C Deogade
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- "There was an irreversible inhibition of CD13/aminopeptidase N (APN) caused by curcumin " s binding ability to APN (Shim et al, 2003)). Several investigators (Arbiser et al, 1998; Dorai et al, 2001) reported in their in vivo studies that curcumin inhibits proliferation and angiogenesis of LNCaP prostate cancer cells. cDNA microarray analysis showed that curcumin inhibits cell cycle progression of endothelial cells by upregulating cyclin-depenent kinase inhibitor (Park et al, 2002). "
ABSTRACT: Numerous treatment modalities are available for variety of systemic and dental diseases; however, the main drawback of conventional medicinal therapies is its various side effects causing harm to the patient. This diverted the interest of researchers towards an alternative approach where natural compounds derived from plants could be used for treating those patients. Curcumin is such an alternative which exhibits a number of medicinal properties and has been used from the centuries. This article discusses the efficacy of curcumin in maintenance of oral health, in particular, and overall health, in general. Curcumin (diferuloylmethane) is a polyphenol derived from the curcuma longa plant, commonly known as turmeric, is a herb known for its medicinal properties. It has a variety of therapeutic properties like anti-inflammatory, anti-oxidant, anti-microbial, hepatoprotective, immunostimulant, antiseptic , anti-angiogenic, apoptotic and anti-mutagenic. All these beneficial properties makes this compound quite more useful in dental field especially in treating periodontal diseases and cancers involving head and neck region and oral cavity. It can also be formulated as a pit and fissure sealant, mouth wash, and subgingival irrigant in different preparations in different dosages and also as a component in local drug delivery system in gel form. INTRODUCTION Most of the anti-cancer drugs are very toxic, highly inefficient for cancer treatment or highly expensive. Thus, these drugs show their limited potential in cancer therapy and beyond the reach of the majority of affected individuals. Hence, an alternative medicinal product without exhibiting such drawbacks will be good option as an anti-cancer drug. Natural phytochemicals isolated from plants used as traditional medicines are referred as such sources. Curcumin, derived from the dried rhizome of the East Indian turmeric plant (Curcuma longa), a perennial herb belonging to the Zingiberacae (ginger) family of botanicals, has drawn attention as alternative source in cancer therapy. This turmeric plant is 3 feet in height and Corresponding AuthorInternational Journal of Biological & Pharmaceutical Research 01/2015; 2015(64):281-290.
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- "Curcumin has shown an apoptotic effect by inhibiting various genes such as protein tyrosine kinase, protein kinase C, c-myc mRNA expression, and bcl-2 mRNA expression  and also mitochondrial pathway. Earlier studies have shown that curcumin possesses an apoptotic activity in different types of cancer cell such as human colon cancer cells, stomach, and skin tumors, breast cancer cells, and prostate cancer cells [44–47]. Study of colon cancer cell line showed that apoptosis was increased in response to curcumin [48, 49]. "
ABSTRACT: Cancer is the most dreadful disease worldwide in terms of morbidity and mortality. The exact cause of cancer development and progression is not fully known. But it is thought that cancer occurs due to the structural and functional changes in the genes. The current approach to cancer treatment based on allopathic is expensive, exhibits side effects; and may also alter the normal functioning of genes. Thus, a safe and effective mode of treatment is needed to control the cancer development and progression. Some medicinal plants provide a safe, effective and affordable remedy to control the progression of malignant cells. The importance of medicinal plants and their constituents has been documented in Ayurveda, Unani medicine, and various religious books. Curcumin, a vital constituent of the spice turmeric, is an alternative approach in the prevention of cancer. Earlier studies have shown the effect of curcumin as an antioxidant, antibacterial, antitumor and it also has a noteworthy role in the control of different diseases. In this review, we summarize the understanding of chemopreventive effects of curcumin in the prevention of cancer via the regulation of various cell signaling and genetic pathways.BioMed Research International 09/2014; 2014:761608. DOI:10.1155/2014/761608 · 2.71 Impact Factor
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- "This proprietory blend of curcumin (under US patent # 5,861,415) was used in a synthetic test diet for SCID mice, specially prepared by Purina Test Diets, Inc. The maximum concentration of curcumin in the diet was fixed at 1% and 2% on the basis of our preliminary in vivo studies on nude mice using LNCaP cells . These doses were tolerated by mice very well without any untoward toxicity, weight loss or morbidity. "
ABSTRACT: A number of studies have focused on the beneficial properties of Curcumin (diferuloyl methane, used in South Asian cuisine and traditional medicine) such as the chemoprevention of cancer. Recent studies have also indicated that this material has significant benefits for the treatment of cancer and is currently undergoing several clinical trials. We have been interested in the application of this compound as a therapeutic agent for advanced prostate cancer, particularly the skeletal complications in this malignancy. Our earlier work indicated that this compound could inhibit the osteomimetic properties which occur in castration resistant prostate cancer cells, by interfering with the common denominators between these cancer cells and the bone cells in the metastatic tumor microenvironment, namely the osteoblasts and the osteoclast. We predicted that curcumin could break the vicious cycle of reciprocal stimulation that results in uncontrolled osteolysis in the bony matrix. In this work, we have evaluated the potential of this compound in inhibiting the bone metastasis of hormone refractory prostate cancer cells in an established animal model. Our results strongly suggest that curcumin modulates the TGF-β signaling that occurs due to bone matrix degradation by up-regulating the metastasis inhibitory bone morphogenic protein-7 (BMP- 7). This enhancement of BMP-7 in the context of TGF-βin the tumor microenvironment is shown to enhance the mesenchymal-to-epithelial transition. Most importantly, we show that as a result of BMP-7 up-regulation, a novel brown/beige adipogenic differentiation program is also up-regu- lated which plays a role in the inhibition of bone metastasis. Our results suggest that curcumin may subvert the TGF-βsignaling to an alternative adipogenic differentiation program in addition to the previously established interference with the osteomimetic properties, thus inhibiting the bone metastatic processes in a chemopreventive as well as therapeutic setting.Journal of Cancer Therapy 04/2014; 5(4):369-386. DOI:10.4236/jct.2014.54044