Automated solid-phase extraction for sample preparation followed by high-performance liquid chromatography with diode array and mass spectrometric detection for the analysis of resveratrol derivatives in wine.
ABSTRACT A method has been developed for the simultaneous determination of resveratrol in all its forms (free isomers and glycosylates) in wines by high-performance liquid chromatography with diode array and mass spectrometric detection. Prior to injection into the column, preconcentration of the sample by automated solid-phase extraction is carried out. In the detection by UV absorption, quantitation was carried out at 280 and 305 nm, and in detection by mass spectrometry, quantitation was performed in the selected ion monitoring mode at m/z 228 and at m/z 238. A comparative study between both detection systems was carried out.
Article: Resveratrol in Peanuts.[Show abstract] [Hide abstract]
ABSTRACT: Peanuts are important dietary food source of resveratrol with potent antioxidant properties implicated in reducing risk of cancer, cardiovascular and Alzheimer's disease, and delaying aging. Resveratrol is a naturally occurring stilbene phytoalexin phenolic compound produced in response to a variety of biotic and abiotic stresses. This paper is a review of trans-resveratrol and related stilbenes from peanuts-their chemical structures, mechanisms for their biosynthesis, and concentrations in comparison with other major food sources. It will also discuss trans-resveratrol's absorption, bioavailability, and major health benefits; processes to enhance their biosynthesis in peanuts by biotic and abiotic stresses; process optimization for enhanced levels in peanuts and their potential food applications; and methods used for its extraction and analysis.Critical reviews in food science and nutrition 01/2014; 54(6):734-770. · 3.73 Impact Factor
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ABSTRACT: trans-Resveratrol(3,5,4'-trihydroxystilbene) is phenolic compound present in grapes, wines, and peanuts, has been reported to have health benefits including anticarcinogenic effects, protection against cardiovascular diseases and reduced cancer risk. A simple method for the quantitative extraction of trans-resveratrol from peanut has been developed. Optimal conditions for extraction were investigated. Type of solvent, time, and temperature assayed influenced trans-resveratrol yield. Adequate extraction condition was decided to ethanol/water (80:20v/v) maintained at for 45 min. After extraction, the protocol consists of sample preparation using a solid-phase extraction (SPE) cartridge after concentrate with rotary evaporator and quantified by reversed phase HPLC using a column at 308 nm. Analytical methods for measuring trans-resveratrol in peanut were adapted to isolate, identify, and quantify trans-resveratrol in 11 peanut varieties by HPLC (high-performance liquid chromatography) with UV detector, The 11 peanut varieties content ranged from 0.018 to 1.125 with an average of 0.289 . The contents were higher in the seeds with than without testa, regardless of varieties. The trans-resveratrol content was Higher in 110, 130 days after sowing than that of other period.Korean Journal of Crop Science. 01/2003; 48(6).
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ABSTRACT: form only given. We report the results of temperature (T) dependent dc conductivity (/spl sigma//sub dc/), thermopower (S), microwave dielectric constant (E/sub mw/ and EPR studies for camphor sulfonic acid doped polyaniline (PAN-CSA) and its methyl ring-substituted derivative poly(o-toluidine) (POT-CSA) fibers, and contrast them with HCI doped samples (PAN-HCI and POT-HCI). For POT-HCl fibe, /spl sigma//sub dc/(295K) ~ 0.02 S/cm and /spl sigma//sub dc/(T) x exp[-(T/sub o/(/T)/sup -1/2/] with T/sub o//spl cong/3.8 x 10/sup 4/ K, very similar POT-HCl powder./sup 1/ In contrast, for POT-CSA fiber, /spl sigma//sub dc/(295K) ~ 10 S/cm and /sol sigma//sub dc/(T) shows much weaker T dependence with an effective T/sub o/ //spl cong/ 350 K. POT-CSA fiber also shows much higher /spl epsi//sub mw/ (T) and dramatically different thermopower than POT-HCl with S = A + BT, where A 0, indicating that POT-CSA fiber is much closer to localization-delocalization boundary than POT-HCl. The results for PAN-CSA fiber are very similar to those for PAN-CSA films/sup 2/ with the presence of some poorer conducting linkages. Supported in part by OSU Center for Materials Research and US ONR01/1994;