Entire genome sequence analysis of the Hantavirus Z10 strain, China

National Institute for the Control of Pharmaceutical and Biological Pruducts, Beijing 100050, China.
Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology 07/2001; 15(2):112-5.
Source: PubMed


Study on the complete genome sequence of Hantavirus Z10 strain which has been applied for inactivated vaccine production in China, to assess its molecular characteristics and the diversity with other hantaviruses.
The total RNA were prepared from Z10 virus infected cells and the RT-PCR products was cloned into T vector, sequenced and analyzed by using DNASTAR software.
The Z10 complete genome, L segment is 6,553, M segment is 3,615, S segment is 1,701 nucleotides in length, with a single open reading frame encoding 2,151, 1,135, 429 amino acids respectively. Sequence homology comparison showed that the 3 segment nucleotide of Z10 strain were close to HTN type virus, but only 83.6-87.4% homology with other HTN viruses at the nucleotide level. The phylogenetic analysis was made on their nucleotide and amino acid sequences.
The results firstly demonstrates that Z10 strain is a new subtype of the Hantaan(HTN) type.

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    • "In contrast to the topology of the S tree, all HTNV isolates formed seven distinct phylogroups and showed monophyletic ancestry in the tree constructed from the M segment sequences (Fig. 1b). Furthermore, strains Z5 (EF103195) and Z10 (Yao et al., 2001b) formed group M7, which was different from the other S3 viruses, although there was only weak bootstrap support for the M7 group. "
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