Indole alkaloids from the seeds of Centaurea cyanus (Asteraceae).
ABSTRACT Preparative RP-HPLC analysis of a methanol extract of the seeds of Centaurea cyanus afforded four indole alkaloids: moschamine, cis-moschamine, centcyamine and cis-centcyamine, the latter two being new natural products. Structures of these compounds were elucidated by comprehensive spectroscopic analyses. General toxicity of the isolates was determined by Brine Shrimp Lethality bioassay.
- SourceAvailable from: scielo.org.coInfectio. 09/2010; 14(3):186-194.
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ABSTRACT: Natural products from plant sources, embracing inherently ample structural diversity than synthetic ones are the major sources of anticancer agents and will constantly play as protagonists for discovering new drugs. Polo-like kinases (PLKs) play a leading role in the ordered execution of mitotic events and 4 mammalian PLK family members have been identified. PLK1 is an attractive target for anticancer drugs in mammalian cells, among the four members of PLKs. The present study expresses the molecular interaction of compounds (1,2-Benzenedicarboxylic acid bis (2 ethylhexyl) ester, squalene, 3,5-bis (1,1-dimethylethyl) phenol, Pentamethyl tetrahydro-5H-chromene, (1,4-Cyclohexylphenyl) ethanone and 6-Vinyl-7-methoxy-2,2-dimethylchromene) isolated from methanolic extract of leaves of Ageratum houstonianum with PLK1 enzyme. Docking between PLK1 and each of these compounds (separately) was performed using "Auto dock 4.2." (1,4-Cyclohexylphenyl) ethanone showed the maximum potential as a promising inhibitor of PLK1 enzyme with reference to ∆G (-6.84 kcal/mol) and Ki (9.77 μM) values. This was sequentially followed by Pentamethyl tetrahydro-5H-chromene (∆G = -6.60 kcal/mol; Ki = 14.58 μM), squalene (∆G = -6.17 kcal/mol; Ki = 30.12 μM), 6-Vinyl-7-methoxy-2,2-dimethylchromene (∆G = -5.91 kcal/mol; Ki = 46.68 μM), 3, 5-bis (1,1-dimethylethyl) phenol (∆G = -5.70 kcal/mol; Ki = 66.68 μM) and 1,2-Benzenedicarboxylic acid bis (2 ethylhexyl) ester (∆G = -5.58 kcal/mol; Ki = 80.80 μM). These results suggest that (1,4-Cyclohexylphenyl) ethanone might be a potent PLK1 inhibitor. Further, in vitro and in vivo rumination are warranted to validate the anticancer potential of (1,4-Cyclohexylphenyl) ethanone.Pharmacognosy Magazine 01/2014; 10(Suppl 1):S14-21. · 1.53 Impact Factor
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ABSTRACT: Essential oil composition of Centaurea kilaea flowers, stems and C. cuneifolia flowers were investigated by GC, GC/MS. Flowers and stems of C. kilaea afforded very low essential oil yield <0.01% (v/w). Nineteen and twenty compounds were identified in the flower and stem oils which represent 59.5% and 77.6% of the C. kilaea oil respectively. The main components of the C. kilaea flower oil were hexadecanoic acid 26.2%, tetradecanoic acid 18.1%, β-Eudesmol 3.3% and decanoic acid 3.1%. The stem oil contains hexadecanoic acid 55.5% and β-Eudesmol 3.2%. Flowers of C. cuneifolia afforded very low essential oil yield <0.01% (v/w). Twenty five compounds were identified in the flower oil of C. cuneifolia which represent 69.92% of the oil. The main components of this oil include hexadecanoic acid 32.9%, tetradecanoic acid 14.4%, heptacosane 6.1% and nonacosane 4.3%. Essential oils obtained from both species have saturated fatty acids and alkanes as the major components, but both oils also contain minor amounts of sesquiterpenes.Natural Volatiles & Essential Oils. 12/2014; 1(1):55-59.