Polyamine distribution profiles in the eighteen genera phylogenetically located within the Flavobacterium–Flexibacter–Cytophaga complex

School of Health Sciences, Faculty of Medicine, Gunma University, Maebashi, Japan.
Microbios 02/2001; 106(413):7-17.
Source: PubMed

ABSTRACT Cellular polyamines of eighteen genera belonging to the Flavobacterium-Flexibacter-Cytophaga complex were analysed by ion exchange liquid chromatography. Homospermidine was the major polyamine in the genera Bergeyella, Riemerella, Ornithobacterium, Weeksella, Capnocytophaga, Polaribacter and Psychroflexus belonging to the family Flavobacteriaceae. In the family Spirosomaceae, Runella, Spirosoma and Flectobacillus species contained spermidine whereas Cyclobacterium species contained homospermidine. Within a divergent cluster, Haliscomenobacter and Lewinella species contained spermidine whereas Saprospira grandis contained agmatine alone. The major polyamine of Chitinophaga and Sporocytophaga species was homospermidine. Flexithrix dorotheae contained spermidine. Microscilla marina, the type species of the genus Microscilla, contained spermidine and cadaverine. However, 'Microscilla sericea' contained homospermidine, 'Microscilla furvescens' contained spermidine, and 'Microscilla arenaria' lacked all polyamines. Polyamine profiles serve as a phenotypic chemotaxonomic marker for the reclassification of the genera belonging to the complex.

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    • "The Cytophaga^Flavobacteria cluster belongs to a diverse bacterial division that has been labeled di¡erently over the years. Hugenholtz et al. [1] and other authors have used`Cytophagales' while Hamana and Nakagawa [4] recently stated that the division name was`Flavobacterium^Flexibacter^Bacteriodes^Cytophaga'. The latter name does give some indication of the diversity of this division and highlights some of the major genera (Fig. 1). "
    FEMS Microbiology Ecology 03/2002; 39(2):91-100. DOI:10.1111/j.1574-6941.2002.tb00910.x · 3.88 Impact Factor
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    ABSTRACT: Cellular polyamines of the newly additional 19 species belonging to the class Bacteroides of the phylum Bacteroidetes were analyzed by HPLC to display polyamine distribution as a chemotaxonomic marker within the total 41 species. Three profiles, the presence of spermidine, the presence of homospermidine and the absence of both triamines, corresponded to their phylogenetical positions within the four families of the class. The occurrence of an aromatic amine, 2-phenylethylamine, extracted into cellular polyamine fraction, was also determined within the 121 species distributed within the phylum. This aromatic amine was found in Cellulophaga lytica, Cytophaga latercula, Tenacibaculum amylolyticum, Tenacibaculum martimum, Tenacibaculum mesophilum and Psychroflexus torquis belonging to the family Flavobacteriaceae of the class Flavobacteria, and Flexibacter flexilis and Microscilla marina belonging to the family Flexibacteraceae of the class Sphingobacteria.
    The Journal of General and Applied Microbiology 11/2004; 50(5):255-60. DOI:10.2323/jgam.50.255 · 0.60 Impact Factor
  • 12/2005: pages 455-480;
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