Polyamine distribution profiles in the eighteen genera phylogenetically located within the Flavobacterium–Flexibacter–Cytophaga complex

School of Health Sciences, Faculty of Medicine, Gunma University, Maebashi, Japan.
Microbios 02/2001; 106(413):7-17.
Source: PubMed


Cellular polyamines of eighteen genera belonging to the Flavobacterium-Flexibacter-Cytophaga complex were analysed by ion exchange liquid chromatography. Homospermidine was the major polyamine in the genera Bergeyella, Riemerella, Ornithobacterium, Weeksella, Capnocytophaga, Polaribacter and Psychroflexus belonging to the family Flavobacteriaceae. In the family Spirosomaceae, Runella, Spirosoma and Flectobacillus species contained spermidine whereas Cyclobacterium species contained homospermidine. Within a divergent cluster, Haliscomenobacter and Lewinella species contained spermidine whereas Saprospira grandis contained agmatine alone. The major polyamine of Chitinophaga and Sporocytophaga species was homospermidine. Flexithrix dorotheae contained spermidine. Microscilla marina, the type species of the genus Microscilla, contained spermidine and cadaverine. However, 'Microscilla sericea' contained homospermidine, 'Microscilla furvescens' contained spermidine, and 'Microscilla arenaria' lacked all polyamines. Polyamine profiles serve as a phenotypic chemotaxonomic marker for the reclassification of the genera belonging to the complex.

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    • "The Cytophaga^Flavobacteria cluster belongs to a diverse bacterial division that has been labeled di¡erently over the years. Hugenholtz et al. [1] and other authors have used`Cytophagales' while Hamana and Nakagawa [4] recently stated that the division name was`Flavobacterium^Flexibacter^Bacteriodes^Cytophaga'. The latter name does give some indication of the diversity of this division and highlights some of the major genera (Fig. 1). "

    FEMS Microbiology Ecology 03/2002; 39(2):91-100. DOI:10.1111/j.1574-6941.2002.tb00910.x · 3.57 Impact Factor
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    ABSTRACT: Cellular polyamines of 58 strains belonging to the Flavobacterium-Flexibacter-Cytophaga-Sphingobacterium complex were analysed by HPLC. Homospermidine was found in all species of Flavobacterium, Chryseobacterium, Empedobacter, Myroides, Cellulophaga, Salegentibacter, Psychroserpens and Gelidibacter of the family Flavobacteriaceae. Flavobacterium ferrugineum located outside of this family also contained homospermidine. Cytophaga fermentans and C. xylanolytica belonging to the family Bacteroidaceae contained spermidine. Cytophaga marinoflava and C. latercula belonging to Flavobacteriaceae contained homospermidine. The Cytophaga hutchinsonii/C. aurantiaca group contained homospermidine which was the major polyamine in Flexibacter maritimus/ F. ovolyticus of the family Flavobacteriaceae. The Flexibacter sancti/F filiformis/ Cytophaga arvensicola group, F. elegans, F. ruber, F. canadensis, F. flexilis and F. tractuosus, were located separately in different six clusters, and contained homospermidine. The Flexibacter litoralis/F. polymorphus/F. aggregans group contained spermidine, which was detected in Flexibacter roseolus belonging to a divergent cluster. Sphingobacterium and Pedobacter species of the family Sphingobacteriaceae contained homospermidine. Polyamine profiles serve, as a phenotypic chemotaxonomic marker, for the classification of this complex.
    Microbios 02/2001; 106 Suppl 2:105-16.

  • The Journal of General and Applied Microbiology 05/2001; 47(2):103-105. DOI:10.2323/jgam.47.103 · 0.94 Impact Factor
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