Multiplication of Musa from excised stem tips

Department of Biochemistry, Division of Biological Sciences, State University of New York at Stony Brook 11794, USA.
Annals of Botany (Impact Factor: 3.65). 04/1984; 53(3):321-8.
Source: PubMed


Rapidly multiplying cultures were established from excised shoot tips of two dessert banana clones ('Philippine Lacatan' and 'Grande Naine') and two plantain clones ('Pelipita' and 'Saba'). Apices cultured on semi-solid media produced single shoots while apices placed in liquid media produced shoot clusters. Individual shoots were induced to form multiple shoot clusters by longitudinally splitting the shoot through the apex. Shoot multiplication was stimulated maximally by 5 mg l-1 benzylaminopurine. Rooted plants were produced by treating shoots with auxins. Growth rates based on increase in f. wt of the four clones were compared. During a 4-week culture period 'Pelipita' showed a fivefold increase in f. wt while 'Grande Naine', 'Philippine Lacatan' and 'Saba' showed increases exceeding tenfold.

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    • "Microbes like bacteria, fungi and virus are the most common contaminants observed in cultures. To overcome this sterilizing agents with different concentrations are generally used like 0.25% sodium hypochlorite (Vessey and Revera, 1981) chlorine saturated water for 15-20 minutes (Dore et al., 1983), 0.05% sodium hypochlorite (Cronauer and Krikarion, 1984a). "
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    ABSTRACT: ASTRACT The study was undertaken with a view to establish a protocol for in vitro plant regeneration from shoot tip explants of banana. Different concentrations of BAP (0.0, 1.0, 2.0, 3.0, 4.0, 5.0, 6.0 and 7.0 mg/l) and IBA (0.0, 1.0, 2.0, 3.0, 4.0 and 5.0 mg/l) was used in MS medium to assess the influence on in vitro shoot regeneration and subsequent root formation of Amritasagar and Sabri banana cultivars. Maximum single shoot formation (50.0% and 30.0%), number of single shoot (3.50 and 2.00 in), longest shoot (2.64 cm and 2.16 cm) were found from 0.5 mg/l BAP while maximum number of roots (3.83 and 2.50) and longest root (3.60 cm and 3.10 cm) was found from 0.3 mg/l IBA in Amritasagar and Sabri respectively. The survival of the plantlets of both cultivars was more than 82% under ex vitro condition. 0.5 mg/l BAP and 0.3 mg/l IBA can be used with MS media for shoot and root formation of Amritasagar and Sabri banana cultivars through shoot tip culture.
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    • "The excision of an apex is important for mass propagation of valuable ornamental plants with a naturally low rate of multiplication like Strelitzia sp. and Calathea sp. Cronauer and Krikorian [27] also reported the similar findings in induction of multiple shoots of two dessert banana clones and two plantain clones through excision of shoot tips. This phenomenon had been discussed precisely by Shimizu-Sato et al. [28] as it was found that cytokinin will be induced by decapitation of the shoot apex and stimulate axillary bud outgrowth. "
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    ABSTRACT: A successful protocol was established for micropropagation in two selected varieties of exotic ornamental plants, Calathea crotalifera . The effects of different sterilization techniques, explant type, and the combination and concentration of plant growth regulators on shoots induction were studied. The axillary shoot buds explants sprouted from rhizomes in soil free conditions showed high induction rate of shoots with lowest contamination percentage when treated with combination of 30% (v/v) NaOCl, 70% (v/v) ethanol, and 0.3% (w/v) HgCl 2 . In the present study, the highest number of multiple shoots was obtained in MS basal medium supplemented with 3.5 mg/L 6-Benzylaminopurine (BAP), 1.0 mg/L 1-Naphthaleneacetic acid (NAA), 3% sucrose, and 6 g/L plant agar for both varieties and was used as multiplication medium. Microshoots were highly induced when the young shoot bud explants were incised longitudinally prior subculture. Chlorophyll analysis was studied to test the effects of activated charcoal and L-glutamine on reduction of necrosis problem. The maximum roots induction was recorded on MS medium supplemented with 1.0 mg/L 1-Naphthaleneacetic acid (NAA) compared to indolebutyric acid (IBA). The complete regenerated plantlets were successfully acclimatized in the soilless medium under greenhouse condition. This is the first report of rapid mass propagation for C. crotalifera .
    The Scientific World Journal 07/2014; 2014(7). DOI:10.1155/2014/457092 · 1.73 Impact Factor
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    • "This was followed by concentration of 1.5 mg/l while the concentrations of 1.0 mg/l and 0.5 mg/l statistically produced same number of roots per explant. In vitro rooting of banana can be induced by transferring the explants to the basal media alone [26] [27]. Gubbuk and Pekmezci [11], reported that activated charcoal was added to the media in replacement of auxins such as IAA or IBA. "

    American Journal of Plant Sciences 01/2013; 04(11):2174-2180. DOI:10.4236/ajps.2013.411269
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