Multiplication of Musa from excised stem tips.
ABSTRACT Rapidly multiplying cultures were established from excised shoot tips of two dessert banana clones ('Philippine Lacatan' and 'Grande Naine') and two plantain clones ('Pelipita' and 'Saba'). Apices cultured on semi-solid media produced single shoots while apices placed in liquid media produced shoot clusters. Individual shoots were induced to form multiple shoot clusters by longitudinally splitting the shoot through the apex. Shoot multiplication was stimulated maximally by 5 mg l-1 benzylaminopurine. Rooted plants were produced by treating shoots with auxins. Growth rates based on increase in f. wt of the four clones were compared. During a 4-week culture period 'Pelipita' showed a fivefold increase in f. wt while 'Grande Naine', 'Philippine Lacatan' and 'Saba' showed increases exceeding tenfold.
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ABSTRACT: An efficient, simple, and rapid regeneration system was established for bananas (Musa sp.) using sections of corm containing intercalary meristematic tissues as explants. Six different cultivars of banana (‘Mpologoma’, ‘Nakitembe’, ‘Mbwazirume’, ‘Pisang awak’, ‘Sukali ndiizi’, and ‘FHIA-17’) with diverse genetic constitution and ploidy levels were regenerated on Murashige and Skoog (MS) medium supplemented with 5 mgl of 6-benzylaminopurine. All the cultivars showed relatively high regeneration efficiency. About 93–97% of the explants regenerated, producing, on average, 12–13 shoots from whole section and 16–19 shoots in total from quarter pieces of each section. Regeneration from intercalary meristematic tissues offers a simple, efficient method for transformation of a broad range of banana cultivars, including East African Highland bananas.Journal of Crop Improvement 01/2008; 22(2):171-180.
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ABSTRACT: Male inflorescences have potential to be used as explants for rapid micropropagation of Musa sp. The male flowers of four banana cultivars, namely 'Berangan', 'Rastali', 'Nangka' and 'Abu' belonging to three genome types in Musa (AAA, AAB, and ABB), were cultured onto Murashige and Skoog (MS) medium which was supplemented with 1 mg/L of TDZ, BAP, Kin, 2-ip and Zea. The number of shoots was found to significantly increase in both TDZ and BAP treatments, as compared to other cytokinins. TDZ at 0.4, 0.6 and 0.8 mg/L, in particular, appeared to be optimum for shoot induction in 'Berangan-AAA', 'Rastali-AAB' and 'Nangka-AAB' and 'Abu-ABB', respectively. However, all the cultivars showed their highest response to regeneration at 8 mg/L of BAP. After the initiation of the explants onto the MS media for all the cultivars, the highest number of cauliflower-like bodies' (CLBs) clusters was observed at two months of culture. The number of induced 'CLBs' cluster is dependent on the size of male buds. Male inflorescences with the size of 20 mm were found to induce more 'CLBs' clusters. Meanwhile, the number of shoots produced is dependent on both the cytokinins and cultivars used.AFRICAN JOURNAL OF BIOTECHNOLOGY 05/2010; 9:2360-2366. · 0.57 Impact Factor
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ABSTRACT: Two essays were carried out to evaluate the effect of different types of auxins on root formation and the influence of dark and culture substratum on tetraploid hybrid FHIA-01 proliferation (Musa spp. AAAB). The plant material consisted of tissue culture plantlets of FHIA -01 hybrid tetraploid banana (Musa AAAB). The trial, with a total of 10 replicates per treatment was carried out in each pot containing five explants. For both tests, a combination of two cytokinins was enriched in culture substratum. The results obtained show that regeneration was high in culture substratum with light than substratum without meta-methoxytopolin riboside (M2). The medium M2 to the light induced a higher number of the buds compared to medium dose reduced meta-methoxytopolin riboside (M1). Meanwhile, only explants inoculated on the medium M1 in the dark induced callus. The bud proliferation, induction of root, leaf and the broadcast callus induction are significantly influenced by the different substratum and photoperiod, increasing the explant size, the number of emerged leaves, roots and the number of the weight of explant with buds proliferated. Formulating specific culture media cultivars according to group (ABB or AAA) and the choice of culture conditions (light intensity) would avoid consecutive failures and low proliferation in in vitro culture.International journal of innovation and applied studies. 03/2014; 5(3):280-293.