Endoscopic fluorescence spectroscopy in the upper GI tract for the detection of GI cancer: initial experience
ABSTRACT The aim of this study was to investigate autofluorescence spectroscopy using violet-blue excitation light for the in vivo diagnosis of GI cancer during routine endoscopy.
Fluorescence spectra were obtained from normal mucosa and cancerous lesions of the esophagus and stomach. The spectroscopic system used comprised a special light source capable of delivering either white or violet-blue light to induce autofluorescence of tissue via the endoscope. Endogenous fluorescence spectra emitted by the tissue were recorded with a fiberoptic probe and analyzed with a spectrographic detector system consisting of a polychromator with a photodiode array and an optical multichannel analyzer. The data of each spectrum were sampled within the range of 450-700 nm and stored in a personal computer.
Esophageal squamous cell cancer, adenocarcinoma of the esophagus, and adenocarcinoma of the stomach show specific differences in the emitted fluorescence spectra compared with normal mucosa.
Light-induced fluorescence spectroscopy might be a useful tool for the endoscopic in vivo detection of dysplasia and early carcinoma in the upper GI tract. Further trials are needed to test the validity of this new optical detection system.
Evidence-based Oncology 09/2002; 3(3):106-107. DOI:10.1054/ebon.2002.0039
Techniques in Gastrointestinal Endoscopy 01/2010; 12(1):2-11. DOI:10.1016/j.tgie.2010.01.002
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ABSTRACT: Screening and surveillance can prevent gastrointestinal cancer by detecting precursor lesions of cancer, but in many cases dysplasia is invisible to the eye of the endoscopist. Therefore, surveillance in these situations requires extensive random biopsies and histologic examination of the excised tissue for dysplasia. This biopsy strategy may overlook areas of dysplasia and is limited by sampling errors. Spectroscopic methods have the potential to overcome these limitations by rapidly and safely evaluating wide regions of tissue for dysplasia without required excision of the tissue. Spectroscopy can objectively quantify the color and brightness of light and uses this information to detect changes within the mucosa that are too subtle to be appreciated by the naked eye. Most spectroscopic techniques are initially developed and tested with optical fiber probes. These probes have several advantages including ease of passage through the accessory channel of standard diagnostic endoscopes and highly predictable geometry between fibers that provide the source of light and those that deliver collected light to the detector. These factors make point probes highly suitable for research and technology development; however, they are limited by the small surface area they examine at the tip of the probe.Techniques in Gastrointestinal Endoscopy 04/2003; 5(2):82-88. DOI:10.1053/tgie.2003.50002