Multiple Roles of Neurotrypsin in Tissue Morphogenesis and Nervous System Development Suggested by the mRNA Expression Pattern

Institute of Anatomy, University of Zurich, Zurich, CH-8057, Switzerland.
Molecular and Cellular Neuroscience (Impact Factor: 3.84). 11/2001; 18(4):407-33. DOI: 10.1006/mcne.2001.1029
Source: PubMed


We have mapped the spatio-temporal expression of the multidomain serine protease neurotrypsin in the developing mouse by in situ hybridization. On embryonic day (E) 8, mRNA is detected in giant trophoblast cells, later in embryonic mesenchymal tissues. On E11, expression begins in Schwann cell precursors, olfactory epithelium, trigeminal ganglion, and midbrain. The floor plate shows strong expression on E12. Further prenatal development is characterized by rising neurotrypsin mRNA in sensory ganglia and motor neurons. Staining in cerebral cortex emerges around birth and culminates toward the end of the first week with a complex laminar and areal pattern. Expression in peripheral nerves and nonneural tissues vanishes soon after birth and the adult neuronal distribution is gradually established until weaning age. This developmental expression pattern suggests roles of neurotrypsin in morphogenesis of nonneural tissues, as well as in neural development, in particular in axonal target invasion, synaptogenesis, and Schwann cell differentiation.

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    • "In contrast, motopsin is detected in the dendrites or the somatic body of neurons under normal expression levels (Mitsui et al., 2007). The expression of motopsin mRNA in the cerebral cortex culminates around the second week after birth (Iijima et al., 1997; Wolfer et al., 2001), then gradually decreases through the lifetime of an individual. This temporal expression pattern raises the additional possibility that motopsin may be involved in the development of the cerebral cortex. "
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    Cell Biology International 01/2014; 38(1). DOI:10.1002/cbin.10164 · 1.93 Impact Factor
    • "Neurotrypsin is secretion of neuronal cells in various brain regions, like hippocampus, cerebral cortex, and the cranial nerve nuclei.[1012] The mRNA of neurotrypsin is expressed most abundantly during the first postnatal week in the hippocampus and the cingulated cortex but its expression gradually decreases and still continues into adult life.[1213] The abundant expression of neurotrypsin mRNA During the perinatal period is observed in other regions as well, like olfactory system, cranial nerve nuclei, spinal cord, and peripheral nervous system, which suggest that neurotrypsin plays multiple roles in the developing nervous system.[14] "
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    Indian Journal of Human Genetics 03/2011; 17(2):65-9. DOI:10.4103/0971-6866.86178
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    • "PRSS12 (MIM: 606709), also known as neurotrypsin, was the first of such genes to be identified (Molinari et al. 2002). It encodes a trypsin-like serine protease, which is expressed in the embryo, and is likely involved in synapse maturation and neural plasticity (Molinari et al. 2002; Gschwend et al. 1997; Wolfer et al. 2001). It functions in the proteolytic cleavage of agrin at the synapse, which requires postsynaptic NMDAR activation (Matsumoto-Miyai et al. 2009; Stephan et al. 2008; Reif et al. 2007). "
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    Journal of Neurodevelopmental Disorders 12/2010; 2(4):182-209. DOI:10.1007/s11689-010-9055-2 · 3.27 Impact Factor
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