Article

Neosporosis in bovine dairy herds from the west of France: detection of Neospora caninum DNA in aborted fetuses, seroepidemiology of N-caninum in cattle and dogs

Laboratoire Départemental Frank Duncombe, 1 Route de Rosel, 14053 Cedex, Caen, France.
Veterinary Parasitology (Impact Factor: 2.55). 01/2002; 102(4):269-77. DOI: 10.1016/S0304-4017(01)00544-1
Source: PubMed

ABSTRACT Neospora caninum is considered one of the major causes of abortion in cattle in most parts of the world. In this study, the role of N. caninum was investigated in groups of aborted cattle and dairy herds from the west of France. Good correlation was found between parasite DNA detection in fetuses and serologic statuses of dams. In groups with documented abortion status and no antibodies to other pathogens, 17-45% of aborted animals were seropositive for N. caninum, and significant relationship between prevalence of Neospora antibodies and frequency of abortions was found. Neospora-associated abortions were observed all the year round, with a peak in summer. Higher ratios of seropositive abortions were found before the 6th month of gestation. In 12 herds studied in the field, serologic prevalence ranged 6-47%. No difference in age was found between seropositive and seronegative cows. Results indicate that N. caninum is an important and stable cause of abortion in cattle in France.

0 Followers
 · 
52 Views
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: may not store these pages in any form except for your own personal use. All other usage or distribution is illegal under international copyright treaties. Permission to use any of these pages in any other way besides the before mentioned must be gained in writing from the publisher. This article is exclusively copyrighted in its entirety toOJVR.This article may be copied once but may not be, reproduced or re-transmitted without the express permission of the editors. This journal satisfies the refereeing requirements (DEST) for the Higher Education Research Data Collection (Australia). Linking:To link to this page or any pages linking to this page you must link directly to this page only here rather than put up your own page. ABSTRACT Khordadmehr M, Hosseini SMH. Mohsenifar E, Namavari MM, Khordadmehr S., Seroprevalence of Neospora caninum in farm and household dogs determined by ELISA, Online J Vet Res., 16 (4):172-181, 2012. Neospora caninum is an important cause of abortion in dairy cattle worldwide. The dog is the definitive host for N.caninum which also infects dairy cattle. We report seroevalence of Neospora caninum infection in dogs in Fars province in Iran. Serum samples (180) were collected from dogs of different sex, age, and breed and subjected to a modified agglutination (MAT) and standardized dot-ELISA test. Fifty nine samples (54.62%) were positive for N. caninum antibody by dot-ELISA and 49 samples (44.44%) were positive by MAT in titers of 1:100 and 1:80, respectively. There were no significant differences in serological values between different groups of age, sex, household or farm dogs. There was 79.8% agreement between dot-ELISA and MAT tests.
  • [Show abstract] [Hide abstract]
    ABSTRACT: The seroprevalence, in dairy cattle, of antibodies to Neospora caninum, the relationship between seropositivity and age (heifer versus cow), the relationship of herd infection with herd size and the relationship of herd infection with the presence of dogs on the farm were studied. The study involved 549 cows and 82 dogs in 59 dairy herds in Nakhon Pathom, Thailand. A competitive enzyme-linked immunosorbent assay (cELISA) with NC-specific monoclonal antibody was used to detect the NC antibodies in the sera. Individual and herd seroprevalence of NC were 5.5% (30/549) and 34% (20/59), respectively. No significant relationships between NC seropositivity with the age of the cows (heifer versus cow; P>0.05) and between herd infection and the presence of dogs on the farm (P>0.05) were found. Herd size significantly affected herd infection (P<0.05) with higher infection in large than small herds (≥21 versus ≤20 cows). Of 12 cows with a history of abortion, one was seropositive to NC. The seroprevalence of NC antibodies in dogs was 1.2% (1/82). This is the first NC seroprevalence study in dogs in Thailand. It was concluded that Neospora infection was more common at the herd level rather than the individual level in Thailand and the presence of dogs on the farm was not related to the level of herd infection. Caution should be taken in the interpretation of serological tests from the farm dogs.
    Veterinary Parasitology 05/2004; 121(3-4):255-263. DOI:10.1016/S0304-4017(04)00040-8 · 2.55 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: may not store these pages in any form except for your own personal use. All other usage or distribution is illegal under international copyright treaties. Permission to use any of these pages in any other way besides the before mentioned must be gained in writing from the publisher. This article is exclusively copyrighted in its entirety toOJVR.This article may be copied once but may not be, reproduced or re-transmitted without the express permission of the editors. This journal satisfies the refereeing requirements (DEST) for the Higher Education Research Data Collection (Australia). Linking:To link to this page or any pages linking to this page you must link directly to this page only here rather than put up your own page. ABSTRACT Khordadmehr M, Hosseini SMH. Mohsenifar E, Namavari MM, Khordadmehr S., Seroprevalence of Neospora caninum in farm and household dogs determined by ELISA, Online J Vet Res., 16 (4):172-181, 2012. Neospora caninum is an important cause of abortion in dairy cattle worldwide. The dog is the definitive host for N.caninum which also infects dairy cattle. We report seroevalence of Neospora caninum infection in dogs in Fars province in Iran. Serum samples (180) were collected from dogs of different sex, age, and breed and subjected to a modified agglutination (MAT) and standardized dot-ELISA test. Fifty nine samples (54.62%) were positive for N. caninum antibody by dot-ELISA and 49 samples (44.44%) were positive by MAT in titers of 1:100 and 1:80, respectively. There were no significant differences in serological values between different groups of age, sex, household or farm dogs. There was 79.8% agreement between dot-ELISA and MAT tests.