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Neosporosis in bovine dairy herds from the west of France: Detection of Neospora caninum DNA in aborted fetuses, seroepidemiology of N. caninum in cattle and dogs

Laboratoire Départemental Frank Duncombe, 1 Route de Rosel, 14053 Cedex, Caen, France.
Veterinary Parasitology (Impact Factor: 2.55). 01/2002; 102(4):269-77. DOI: 10.1016/S0304-4017(01)00544-1
Source: PubMed

ABSTRACT Neospora caninum is considered one of the major causes of abortion in cattle in most parts of the world. In this study, the role of N. caninum was investigated in groups of aborted cattle and dairy herds from the west of France. Good correlation was found between parasite DNA detection in fetuses and serologic statuses of dams. In groups with documented abortion status and no antibodies to other pathogens, 17-45% of aborted animals were seropositive for N. caninum, and significant relationship between prevalence of Neospora antibodies and frequency of abortions was found. Neospora-associated abortions were observed all the year round, with a peak in summer. Higher ratios of seropositive abortions were found before the 6th month of gestation. In 12 herds studied in the field, serologic prevalence ranged 6-47%. No difference in age was found between seropositive and seronegative cows. Results indicate that N. caninum is an important and stable cause of abortion in cattle in France.

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    • "However, both temperature and humidity likely affect the spread of contagious agents. European studies (Pitel et al., 2001; Carpenter et al., 2006) also reported highest abortion frequencies in summer. "
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    ABSTRACT: Frequency of abortions recorded through Dairy Herd Improvement (DHI) testing was summarized for cows with lactations completed from 2001 through 2009. For 8.5 million DHI lactations of cows that had recorded breeding dates and were >151 d pregnant at lactation termination, the frequency of recorded abortions was 1.31%. Effects of year, herd-year, month, and pregnancy stage at lactation termination; parity; breed; milk yield; herd size; geographic region; and state within region associated with DHI-recorded abortion were examined. Abortions recorded through DHI (minimum gestation of 152 d required) were more frequent during early gestation; least squares means (LSM) were 4.38, 3.27, 1.19, and 0.59% for 152 to 175, 176 to 200, 201 to 225, and 226 to 250 d pregnant, respectively. Frequency of DHI-recorded abortions was 1.40% for parity 1 and 1.01% for parity ≥ 8. Abortion frequency was highest from May through August (1.42 to 1.53%) and lowest from October through February (1.09 to 1.21%). Frequency of DHI-recorded abortions was higher for Holsteins (1.32%) than for Jerseys (1.10%) and other breeds (1.27%). Little relationship was found between DHI-recorded abortions and herd size. Abortion frequencies for effects should be considered to be underestimated because many abortions, especially those caused by genetic recessives, go undetected. Therefore, various nonreturn rates (NRR; 60, 80, …, 200 d) were calculated to document pregnancy loss confirmed by the absence of homozygotes in the population. Breeding records for April 2011 US Department of Agriculture sire conception rate evaluations were analyzed with the model used for official evaluations with the addition of an interaction between carrier status of the service sire (embryo's sire) and cow sire (embryo's maternal grandsire). Over 13 million matings were examined using various NRR for Holstein lethal recessive traits (brachyspina and complex vertebral malformation) and undesirable recessive haplotypes (HH1, HH2, and HH3) as well as >61,000 matings for a Brown Swiss haplotype (BH1), and 670,000 matings for a Jersey haplotype (JH1). Over 80% of fertility loss occurred by 60 d after breeding for BH1, HH3, and JH1, by 80 d for HH2, by 100 d for BY, and by 180 d for HH1. For complex vertebral malformation, fertility loss increased from 40 to 74% across gestation. Association of undesirable recessives with DHI-recorded abortions ranged from 0.0% for Jerseys to 2.4% for Holsteins.
    Journal of Dairy Science 07/2012; 95(7):4074-84. DOI:10.3168/jds.2011-4998
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    • "The wells were then mixed thoroughly by pipetting them up and down several times, covered, and incubated overnight at 37°C with 5% CO2. A cut off titer of 1:80 was considered as significant for the presence of antibodies according to Pitel et al, 2001 and Packham et al, 1998. Reactions were considered positive when the tachyzoites were speared on entire bottom of well of the micro titer plate and those showing button formation were considered negative. "
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    ABSTRACT: may not store these pages in any form except for your own personal use. All other usage or distribution is illegal under international copyright treaties. Permission to use any of these pages in any other way besides the before mentioned must be gained in writing from the publisher. This article is exclusively copyrighted in its entirety toOJVR.This article may be copied once but may not be, reproduced or re-transmitted without the express permission of the editors. This journal satisfies the refereeing requirements (DEST) for the Higher Education Research Data Collection (Australia). Linking:To link to this page or any pages linking to this page you must link directly to this page only here rather than put up your own page. ABSTRACT Khordadmehr M, Hosseini SMH. Mohsenifar E, Namavari MM, Khordadmehr S., Seroprevalence of Neospora caninum in farm and household dogs determined by ELISA, Online J Vet Res., 16 (4):172-181, 2012. Neospora caninum is an important cause of abortion in dairy cattle worldwide. The dog is the definitive host for N.caninum which also infects dairy cattle. We report seroevalence of Neospora caninum infection in dogs in Fars province in Iran. Serum samples (180) were collected from dogs of different sex, age, and breed and subjected to a modified agglutination (MAT) and standardized dot-ELISA test. Fifty nine samples (54.62%) were positive for N. caninum antibody by dot-ELISA and 49 samples (44.44%) were positive by MAT in titers of 1:100 and 1:80, respectively. There were no significant differences in serological values between different groups of age, sex, household or farm dogs. There was 79.8% agreement between dot-ELISA and MAT tests.
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    • "The wells were then mixed thoroughly by pipetting them up and down several times, covered, and incubated overnight at 37°C with 5% CO2. A cut off titer of 1:80 was considered as significant for the presence of antibodies according to Pitel et al, 2001 and Packham et al, 1998. Reactions were considered positive when the tachyzoites were speared on entire bottom of well of the micro titer plate and those showing button formation were considered negative. "
    [Show abstract] [Hide abstract]
    ABSTRACT: may not store these pages in any form except for your own personal use. All other usage or distribution is illegal under international copyright treaties. Permission to use any of these pages in any other way besides the before mentioned must be gained in writing from the publisher. This article is exclusively copyrighted in its entirety toOJVR.This article may be copied once but may not be, reproduced or re-transmitted without the express permission of the editors. This journal satisfies the refereeing requirements (DEST) for the Higher Education Research Data Collection (Australia). Linking:To link to this page or any pages linking to this page you must link directly to this page only here rather than put up your own page. ABSTRACT Khordadmehr M, Hosseini SMH. Mohsenifar E, Namavari MM, Khordadmehr S., Seroprevalence of Neospora caninum in farm and household dogs determined by ELISA, Online J Vet Res., 16 (4):172-181, 2012. Neospora caninum is an important cause of abortion in dairy cattle worldwide. The dog is the definitive host for N.caninum which also infects dairy cattle. We report seroevalence of Neospora caninum infection in dogs in Fars province in Iran. Serum samples (180) were collected from dogs of different sex, age, and breed and subjected to a modified agglutination (MAT) and standardized dot-ELISA test. Fifty nine samples (54.62%) were positive for N. caninum antibody by dot-ELISA and 49 samples (44.44%) were positive by MAT in titers of 1:100 and 1:80, respectively. There were no significant differences in serological values between different groups of age, sex, household or farm dogs. There was 79.8% agreement between dot-ELISA and MAT tests.
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