Article

Bone and bone-marrow interactions: haematological activity of osteoblastic growth peptide (OGP)-derived carboxy-terminal pentapeptide. Mobilizing properties on white blood cells and peripheral blood stem cells in mice.

Department of Oncology, Transplants and Advanced Technologies in Medicine, Hematology Division, University of Pisa, Via Roma 67, 56100, Pisa, Italy.
Leukemia Research (impact factor: 2.92). 02/2002; 26(1):19-27. pp.19-27
Source: PubMed

ABSTRACT Osteogenic growth peptide (OGP) increases blood and bone marrow cellularity in mice, and enhances engraftment of bone marrow transplant. Carboxy-terminal pentapeptide (OGP10-14) holds several properties of full-length polypeptide. We evaluated whether synthetic OGP-derived pentapeptide (sOGP10-14) has some activity on peripheral blood cell recovery after cyclophosphamide-induced aplasia, and on stem cell mobilization. Peripheral blood stem cell (PBSC) mobilization was evaluated by administering granulocyte-colony stimulating factor (G-CSF) or sOGP10-14 after cyclophosphamide (CTX) injection. Haematological parameters and CD34/Sca-1 positive cells were sequentially evaluated. Colony-forming tests were performed in bone marrow cells from CTX-, G-CSF- and sOGP10-14-treated mice. sOGP10-14 was able to enhance band cells and monocyte recovery after cyclophosphamide administration. White blood cell (WBC) counts reached the maximum peak by day +10 but, on day +7, a significant recovery was already detected in sOGP10-14 treated mice. On day +10 the WBC increase in sOGP10-14-treated mice was comparable to that found in G-CSF treated ones. Moreover, CD34/Sca-1 positive early precursors were significantly mobilized by sOGP10-14 compared to the control group. In sOGP10-14-treated mice, the colony-forming unit-granulocyte-macrophage-megakaryocyte (GEMM-CFU) and burst-forming unit-erythroid (BFU-E) were significantly increased in bone marrow cells in comparison to mice treated with CTX only. These results suggest a central role of sOGP10-14 in bone and bone marrow interaction, and a possible role of sOGP10-14 as a mobilizing agent.

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Keywords

administering granulocyte-colony stimulating factor
 
bone marrow cells
 
bone marrow cellularity
 
bone marrow interaction
 
bone marrow transplant
 
Carboxy-terminal pentapeptide
 
CD34/Sca-1 positive cells
 
central role
 
Colony-forming tests
 
cyclophosphamide administration
 
enhances engraftment
 
full-length polypeptide
 
Haematological parameters
 
monocyte recovery
 
Osteogenic growth peptide
 
Peripheral blood
 
peripheral blood cell recovery
 
sOGP10-14-treated mice
 
synthetic OGP-derived pentapeptide
 
White blood cell