Nuclear chromatin characteristics of breast solid pattern ductal carcinoma in situ

Optical Sciences Center, University of Arizona, Tucson, USA.
Analytical and quantitative cytology and histology / the International Academy of Cytology [and] American Society of Cytology (Impact Factor: 0.49). 01/2002; 23(6):418-26.
Source: PubMed


To characterize nuclei from breast solid pattern ductal carcinoma in situ (DCIS) by their karyometric features and to search for the presence of statistically significantly different subsets of nuclei.
One hundred nuclei from each of 6 normal, 13 solid DCIS, (9 low and intermediate grade and 4 high grade DCIS) histopathologic samples of breast tissue were digitally recorded. Karyometric features were computed and subjected to a nonsupervised learning algorithm (P-index) to identify significantly different subgroups.
Nuclei in low grade lesions displayed a diploid/near diploid pattern, while the majority of intermediate grade lesions fell into a range beyond 5N. The high grade lesions showed substantial genomic instability and represented three statistically different subsets or phenotypes.
There is a progression of nuclear abnormality from low grade to high grade DCIS. The nuclei from high grade DCIS form a heterogeneous set that represents three phenotypes. One of these phenotypes shows a nuclear chromatin pattern that more closely resembles poorly differentiated, infiltrating disease. The observation of such a phenotype may have prognostic implications.

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    • "Computer-aided image analysis is such a method. Image analysis has been used to extract quantitative nuclear information useful for diagnosis of biopsy specimens of many tissues, including breast DCIS, and invasive breast (Kerlikowske et al. 2003; Carpenter et al. 1985; Dey et al. 2000; Frank et al. 2001; Hoque et al. 2001; Mariuzzi et al. 1996; Mommers et al. 2001; Susnik et al. 1995; Tuczek et al. 1996; Wolberg et al. 1995). However, these previous reports did not focus on evaluating information for individual nuclei in the classifi cation of patients with mixed nuclear grades. "
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    Modern Pathology 02/2002; 15(1):18-25. DOI:10.1038/modpathol.3880485 · 6.19 Impact Factor
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