Determination of difloxacin and sarafloxacin in chicken muscle using solid-phase extraction and capillary electrophoresis.

Department of Analytical Chemistry, University of Barcelona, Spain.
Journal of Chromatography B (Impact Factor: 2.49). 03/2002; 767(2):313-9. DOI: 10.1016/S1570-0232(01)00587-6
Source: PubMed

ABSTRACT This paper describes a method for residue analysis of difloxacin and sarafloxacin in chicken muscle. Clean-up and preconcentration of the samples are effected by solid-phase extraction (C18) and the determination is carried out by capillary electrophoresis using a photodiode array detection system. The method was validated with satisfying results. The calibration graphs are linear for difloxacin and sarafloxacin from 50 to 300 microg/kg. The limit of detection obtained for difloxacin and sarafloxacin are 10 and 25 microg/kg, respectively, which allows the detection of positive muscle samples at the required maximum residue limits of European Union.