New additions to antibody panels in the characterisation of chronic lymphoproliferative disorders.
ABSTRACT Advances in flow cytometry techniques and the availability of monoclonal antibodies that detect key functional molecules on lymphocytes have contributed greatly to a more precise diagnosis of the chronic lymphoproliferative disorders. In addition to the diagnostic value, the expression of certain markers such as p53 or CD38 provides relevant prognostic information to the clinician. Beyond their diagnostic and prognostic value, immunological markers play a major role in the detection of minimal residual disease, enabling the clinician to estimate more accurately the response to chemotherapy. Those monoclonal antibodies that are relevant to the characterisation of the chronic lymphoproliferative disorders and that could be incorporated in a routine practice are discussed.
Full-textDOI: · Available from: Estela Matutes, Jul 23, 2014
SourceAvailable from: Lacy Cardoso de Brito Junior
Article: Case report of hairy cell leukemia[Show abstract] [Hide abstract]
ABSTRACT: Hairy cell leukemia (HCL) is a rare type of B-cell non-Hodgkin's lymphoma. The clinical symptoms include splenomegaly, pancytopenia, and lymphocytosis. Studies on its carcinogenesis reveal association with exposure to agricultural chemical agents. The objective of this study was to report the case of a male patient, tractor operator, diagnosed with HCL, pancytopenia, cutaneous lesions, without splenomegaly and positive markers for B-cell lymphocytes (CD19, CD20, CD22, CD79b, CD23, Lambda, immunoglobulin M [IgM], CD25 and CD103). Although HCL is a rare disease, late diagnosis may ultimately lead to severe complications and patient's death.Jornal Brasileiro de Patologia e Medicina Laboratorial 12/2011; 47(6):603-606. DOI:10.1590/S1676-24442011000600005
Cytometry Part B Clinical Cytometry 03/2010; · 2.28 Impact Factor
Article: Flow cytometry: Immunophenotyping in 48 hairy cell leukemia cases and the relevance of fluorescence intensity in CDs expression for diagnosis Citometria de fluxo: Imunofenotipagem em 48 casos de leucemia de células cabeludas e a relevância das intensidades de fluorescências nas expressões dos anticorpos monoclonais para o diagnóstico[Show abstract] [Hide abstract]
ABSTRACT: Objectives: To report the experience and the importance of flow cytometry immunophenotyping by measuring the positivity and antigenic expression intensity in the diagnosis of 48 patients with hairy cell leukemia (HCL). Methods: From November 1991 to June 2005, 4318 cases were analyzed by flow cytometry, 3556 (82.3%) of which were oncohematological diseases. Forty-eight cases of hairy cell leukemia (1.3%) were diagnosed. Morphological analysis was performed on slides stained with Grunwald Giemsa panchromatic dye, analyzed by two experienced professionals. The cytochemical analyses made were for acid phosphatase and tartrate-resistant acid phosphatase (TRAP). For antigenic expression analysis, the monoclonal antibodies used were: CD2, CD3, CD5, CD7, CD10, CD11c, CD19, CD20, CD22, CD23, CD25, CD38, HLA-DR, FMC-7, CD79b, CD103, IgM, IgG, IGD, kappa and lambda. results: By analyzing positivity and monoclonal antibody expression intensity in the forward scatter vs. side scatter histograms (used between 1991 and 2001), and in CD19 vs. SSC histograms with sequential histograms (after 2001), it was possible to confirm this pathology and to discriminate residual cells after the specific therapy. Conclusion: Diagnostic confirmation of hairy cell leukemia by flow cytometry is a fast and accurate method that is useful in the clinical laboratory. The option for an initial CD19 vs. SSC histogram and an analysis of antigenic expression intensity in the bone marrow showed to be statistically more efficient.