Intraspecific invariability of the internal transcribed spacer region of rDNA of the truffle Terfezia terfezioides in Europe.
ABSTRACT ITS regions (internal transcribed spacers--ITS1 and ITS2--with the 5.8S gene of the nuclear rDNA) of 25 fruit body samples of Terfezia terfezioides, originating from Hungary and Italy, were compared. The amplification and sequencing of the ITS region was successful with both the ITS1-ITS4 and ITS1F-ITS4 primer pairs. No differences of the restriction fragment length polymorphism profiles were detected among 19 samples collected in one place at the same time. The sequences of the ITS region of 9 samples collected in different localities were highly invariable, differing in only two bases. Thus the intraspecific homogeneity of the ITS region seems to be an important species-specific characteristic of T. terfezioides in contrast to other Terfezia species. As the samples of the species were collected from different and distant localities of Europe, the ITS sequence of T. terfezioides can be considered a very conservative, reliable molecular marker of the fungus.
SourceAvailable from: Rosanne Healy[Show abstract] [Hide abstract]
ABSTRACT: The septal pore ultrastructure of non-ascogenous and reproductive hyphae, as well as development of the spore wall were examined in the truffle Mattirolomyces terfezioides (Mattir.) E. Fisch. (Ascomycota, Pezizales, Pezizaceae) and compared with other pezizalean taxa. The septal pore in ascogenous hyphae of M. terfezioides is a uni- or bi-convex band of the type found in the Pezizaceae. Septal pore ultrastructure in other hyphae included a uniconvex band with lamellate structure. Woronin bodies were globose to hexagonal, and infrequently ellipsoid in shape. Hexagonal Woronin bodies have not been previously reported in the Pezizaceae. Ascospore delimitation starts with two delimiting membranes and the spore-wall development is typical of the Pezizales. The heterogeneously staining spines of the secondary cell wall differ from the structures of Terfezia species. The ultrastructural characteristics of M. terfezioides show the general characteristics of the Pezizaceae, except that the hexagonal Woronin body form is new to this family. The spore delimitation, spine-cap structure, and Woronin body shape of M. terfezioides presented here are in agreement with molecular taxonomic results, which separate the genus from Terfezia and call into question the generic position of Mattirolomyces tiffanyae Healy.Botany 01/2010; 88(1):85-92. DOI:10.1139/B09-094 · 1.04 Impact Factor
Article: MOLECULAR MARKERS IN ECOLOGY[Show abstract] [Hide abstract]
ABSTRACT: The aim of this paper is to encourage reluctant ecologists thinking more on the use of genetic markers. No training in molecular methods is supposed. The resistance may be explained by the confusion of the high number of methods, including laboratory techniques (named by up to four characters) and evaluation. Inevitably, field work needs some extra step to collect DNA source, but sampling strategy is the same or even less restrictive owing to the new, powerful statistical methods. Laboratory techniques develop very fast, many phases can be done automatically and/or many customers provide services on reasonable price. Despite the financial and technical requirements, genetic markers provide high quality information that can be obtained hardly otherwise, or simply impossible. We try to overview the most important genetic markers and technology used recently. Some examples are given by studies of parentage, population structure (migration, fragmentation) or population history.
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ABSTRACT: During revision of Terfezia specimens in the Mycological Collection of the Herbarium of the Real Jardín Botánico of Madrid (MA-Fungi), morphological and molecular analyses revealed a specimen collected in Madrid as the truffle Mattirolomyces terfezioides. This paper presents the first record of the species from the Iberian Peninsula, the western- and southernmost known locality for M. terfezioides in Europe. General characteristics of known collection sites are also discussed.Mycotaxon -Ithaca Ny- 09/2009; 110(1):325-330. DOI:10.5248/110.325 · 0.64 Impact Factor