Isolation, characterization, and fermentative pattern of a novel thermotolerant Prototheca zopfii var. hydrocarbonea strain producing ethanol and CO2 from glucose at 40 degrees C.
ABSTRACT A novel thermotolerant strain of the achlorophyllous micro-alga Prototheca was isolated from a hot spring. The isolate was found to produce an appreciable amount of ethanol and CO2 from glucose under anoxic conditions at both 25 and 40 degrees C; this type of alcohol fermentation has not yet been reported in the genus Prototheca. Moreover, it also evolved gas from sucrose after a time lag at 40 degrees C. Its taxonomic characteristics coincided with those of Prototheca zopfii var. hydrocarbonea, and phylogenetic analysis, based on a small-subunit (SSU) rDNA sequence, also revealed a close relationship between the two strains. D-lactic acid, ethanol, CO2 and a trace of acetic acid were produced from glucose, but L-lactic acid, formic acid, and H2 were not. At 25 degrees C, D-lactic acid and ethanol were produced in approximately equimolar amounts under N2/H2/CO2, whereas ethanol production was predominant under N2. More ethanol was produced at 40 degrees C than at 25 degrees C irrespective of the gas composition in the atmosphere. This is the first report on gas production from glucose and on the changes in the fermentative patterns as a function of temperature for the genus Prototheca.
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ABSTRACT: he patient was a 5-year-old castrated male Maltese. The first visit to the private clinic in Jan 2009 was due to a single lesion of depigmentation and hyperplasia near the ala of nose. Protothecosis was diagnosed by histopathologic evaluation. The patient was treated with itraconazole for about 10 months and died in Dec 2010. Complete necropsy was performed. Multiple ulceration lesions, skin reddish and swollen were found in the hip area and hind legs. In addition, multiple peri- pheral and mesenteric lymph nodes were swollen. Microscopically, the lesions were characterized by pyogranulomatous inflammation with numerous intralesional round to oval, pale stained microorga- nisms infiltrating the subcutaneous, muscle tissues and lymph nodes. With periodic acid-Schiff (PAS) histochemistry, the organisms had characteristic sporangia containing 2 to 4, round sporangiospores with thick cell wall. Skin specimens were also collected for pathogen identification. The pathogen was isolated by colonizing on Sabouraud dextrose agar (SDA) with chloramphenicol. After isolation the pathogen, the DNA extraction was performed by FPFD method and multiplied by PCR method with ￼ ￼ ￼ ￼ spp. 18s rDNA primer. The result was analyzed and compared in NCBI web database (BLAST). Furthermore, the phylogenetic analysis was established through ClustalX (1.81) software for comparison and analyzed by Neighbor-joining method. The result presents this pathogen shows hig- hest similarity with by comparing 18s rDNA. Based on the examination of pathology and molecular biology, cutaneous protothecosis caused by was di- agnosed in this case.Taiwan Veterinary Journal. 03/2013; 39(1):50-58.
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ABSTRACT: The synergetic effects of an immobilized cell system in an oleophilic polyurethane foam (PUF) and use of a thermotolerant strain on degradation of hydrocarbons by colorless green microalga Prototheca zopfii are reported. Two strains of P. zopfii, i.e. thermotolerant RND16 and non-thermotolerant ATCC30253, were immobilized in PUF to compare their abilities to biodegrade a mixed hydrocarbon substrate (MHS) containing aliphatic and polycyclic aromatic hydrocarbons (PAHs), as a function of temperature. The thermotolerant strain RND16 degraded MHS at 35°C, while ATCC30253 did not degrade hydrocarbons at temperatures higher than 30°C. Immobilization of P. zopfii in PUF resulted in shortened lag for growth-associated biodegradation of n-alkanes in MHS, the effect of which was most significant in cultures of RND16 at 25°C. Nevertheless, the decrease in the amount of degraded PAHs was caused by PUF immobilization and the level of this decrease was marked in the cultures of RND16, in which rate and extent of n-alkane degradation were higher than for ATCC30253.Fisheries Science 09/2006; 72(5):1027 - 1033. · 0.90 Impact Factor
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ABSTRACT: The present study screens activity of different en-zymes in Trapa natans under aerobic and anaerobic physiological conditions. Experimental analysis was carried out to assay the activities of ALDH and ADH in the seed extract of T. natans. These enzymes are as-sociated with the alcohol metabolism of the T. natans. It was observed that a metabolic pathway shifts from aerobic respiration to anaerobic fermentation under anoxic conditions. Physiological stress related re-sponse in trapa seeds gives the leads to understand the other model for better fermentable products. During the experiment it was found that there is dif-ference between the concenteration of enzymes in anaerobic and aerobic conditions. Concentration was check with the help of spectrophotometer at 340 nm.Bioscience Biotechnology and Biochemistry 01/2012; 3:304-307. · 1.27 Impact Factor