Article

Ciprofloxacin resistance in Campylobacter jejuni evolves rapidly in chickens treated with fluoroquinolones

Division of Animal and Food Microbiology, Center for Veterinary Medicine, US Food and Drug Administration, Laurel, Maryland 20708, USA.
The Journal of Infectious Diseases (Impact Factor: 5.78). 04/2002; 185(6):837-40. DOI: 10.1086/339195
Source: PubMed

ABSTRACT Fluoroquinolones are commonly used to treat gastroenteritis caused by Campylobacter species. Domestically acquired fluoroquinolone-resistant Campylobacter infection has been documented recently in the United States. It has been proposed that the increase in resistance is due, in part, to the use of fluoroquinolones in poultry. In separate experiments, the effects of sarafloxacin and enrofloxacin treatment of Campylobacter jejuni-infected chickens on the development of ciprofloxacin resistance were measured. Fecal samples were collected before and after treatment and were cultured for C. jejuni. When enrofloxacin or sarafloxacin was used at US Food and Drug Administration-approved doses in broiler chickens, resistance developed rapidly and persisted in C. jejuni. MICs of ciprofloxacin increased from a base of 0.25 microg/mL to 32 microg/mL within the 5-day treatment time frame. These results show that the use of these drugs in chickens rapidly selects for resistant Campylobacter organisms and may result in less effective fluoroquinolone therapy for cases of human campylobacteriosis acquired from exposure to contaminated chicken.

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    • "It is well documented that fluoroquinolone resistance among Campylobacter isolates from human infections increased after the approval of fluoroquinolones in agriculture, especially in poultry farming (Jacobs-Reitsma, Kan, & Bolder, 1994; McDermott et al., 2002). "
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    ABSTRACT: In the present study Campylobacter species and their antimicrobial resistance in Latvian broiler chicken production was determined. Furthermore, this is the first report on the antimicrobial resistance patterns for Campylobacter isolates from broiler chickens at slaughterhouse and retail level in Latvia. Two biggest Latvian broiler chicken meat producing company products were included in the study. Altogether, 74 randomly selected broiler chicken Campylobacter spp. isolates were analysed for species identification. Campylobacter isolates were obtained during a 12-month period within the Latvian Campylobacter prevalence study in 2010. Colony multiplex PCR was used for all isolates to identify Campylobacter species. Minimal inhibitory concentration (MIC) was determined for 58 Campylobacter spp. isolates. Resistance to one or more antimicrobials was detected in all 58 isolates (100%). A high proportion of the isolates were resistant to ciprofloxacin (100%) and nalidixic acid (87.9%). Multidrug resistance, which was determined as resistance to three or more unrelated antimicrobials, was detected in 39 isolates (67.2%). Moreover, all multiresistant isolates were resistant to ciprofloxacin and nalidixic acid. Analyses of Campylobacter isolates from two Latvian broiler chicken meat producing companies resulted with significant differences in Campylobacter species; from the company A mainly Campylobacter coli were found, while in the company B Campylobacter jejuni.
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    • "That is why poultry and poultry products pose also a hazard for potential transfer of (multi)resistant strains to human (Endberg et al., 2001). In this connection, the resistance of Campylobacter spp. to fluoroquinolones and particularly to enrofloxacin, is especially important (Hoge et al., 1998; Mc Dermot et al., 2002; Saenz et al., 2000; Van Den Bogaard and Stobberingh, 1999; Van Looveren et al., 2001). "
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    • "In vitro activities of sarafloxacin against avian E. coli and other infections were studied previously (Soussy et al., 1987, Jones and Erwin, 1998, Medders et al., 1998, Wang et al., 2001 and Smith et al., 2007) and the drug proved its efficacy in comparison with other fluoroquinolones. Sarafloxacin had been proposed successfully for the use in the drinking water of chickens to treat bacterial infections caused by E. coli (McCabe et al., 1993, Charleston et al., 1998, Medders et al., 1998, Hofacre et al., 2000 and Chansiripornchai and Sasipreeyajan, 2002), Salmonella spp (Jiang et al., 2000 and Roy et al., 2002) and Campylobacter jejuni (McDermott et al., 2002) and to prevent spiking mortality in turkeys (Vukina et al., 1998). "
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