The Wright-Giemsa stain - Secrets revealed

Department of Laboratory Medicine and Pathology, University of Minnesota School of Medicine, Minneapolis, Minnesota, USA.
Clinics in Laboratory Medicine (Impact Factor: 1.37). 04/2002; 22(1):15-23. DOI: 10.1016/S0272-2712(03)00065-9
Source: PubMed


The colorful story of the development of the Wright-Giemsa stain is retold. Dramas are replayed, secrets are exposed, and laurels are properly returned to scientists long forgotten. The delicately balanced chemical composition of the stain, once enigmatic, is defined. Finally, an attempt is made to unshroud some of the mystery surrounding the staining method; tried-and-true procedures and useful snippets of laboratory lore are provided. Scientific explanations and reliable methods aside, however, the stain continues to possess a certain mystique, seemingly consisting of equal parts technical expertise, art, and magic.

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