Article

Proton nuclear magnetic resonance characterisation of glycosaminoglycans using chemometric techniques.

Department of Analytical Chemistry, University of Barcelona, Spain.
The Analyst (impact factor: 4.23). 04/2002; 127(3):407-15. pp.407-15
Source: PubMed

ABSTRACT Various types of glycosaminoglycans (GAGs) including heparins, chondroitin sulfates, dermatan sulfate and hyaluronic acid were studied from their proton nuclear magnetic resonance (1H NMR) spectra using chemometric techniques. Despite the complexity of the 1H NMR signals, data analysis using principal component analysis enabled the different GAG classes to be distinguished and permitted their classification according to their chemical structure. The analysis of the composition of the major disaccharide unit and other relevant chemical structures in the heparin samples was performed using partial least squares regression.

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Keywords

1H NMR
 
1H NMR signals
 
chemometric techniques
 
different GAG classes
 
distinguished
 
GAGs
 
glycosaminoglycans
 
hyaluronic acid
 
major disaccharide unit
 
partial
 
principal component analysis
 
proton nuclear magnetic resonance