Guidelines for visualization of cutaneous blood flow by laser Doppler perfusion imaging - A report from the Standardization Group of the European Society of Contact Dermatitis based upon the HIRELADO European community project

Ruhr-Universität Bochum, Bochum, North Rhine-Westphalia, Germany
Contact Dermatitis (Impact Factor: 3.75). 04/2002; 46(3):129-40. DOI: 10.1034/j.1600-0536.2002.460301.x
Source: PubMed


This report reviews how to set up a laser Doppler perfusion imaging system intended for visualization of skin blood perfusion, capture images and evaluate the results obtained. A brief summary of related papers published in the literature within the areas of skin irritant and allergy patch testing, microdialysis and skin tumour circulation is presented, as well as early applications within other fields such as diabetology, wound healing and microvascular research.

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Available from: Chris Anderson, Aug 15, 2015
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    • "The microvasculature reflects the health of the tissue and in particular its metabolic demand and its alteration should therefore exhibit a characteristic signature of the disease. In case of inflammation, we expect an increase in blood perfusion as has been previously demonstrated with Laser Doppler Imaging [19]. Basal cell carcinoma is expected to exhibit increased and chaotic vascularization through neoangiogenesis, as a result of the strong metabolic need due to uncontrolled cell growth. "
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    ABSTRACT: We demonstrate noninvasive structural and microvascular contrast imaging of different human skin diseases in vivo using an intensity difference analysis of OCT tomograms. The high-speed swept source OCT system operates at 1310 nm with 220 kHz A-scan rate. It provides an extended focus by employing a Bessel beam. The studied lesions were two cases of dermatitis and two cases of basal cell carcinoma. The lesions show characteristic vascular patterns that are significantly different from healthy skin. In case of inflammation, vessels are dilated and perfusion is increased. In case of basal cell carcinoma, the angiogram shows a denser network of unorganized vessels with large vessels close to the skin surface. Those results indicate that assessing vascular changes yields complementary information with important insight into the metabolic demand.
    Biomedical Optics Express 10/2012; 3(10):2636-46. DOI:10.1364/BOE.3.002636 · 3.65 Impact Factor
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    • "A small hole allowed the passage of the laser light through the chamber. As recommended (Fullerton et al, 2002), the drug delivery electrode was filled with 180 ml of acetylcholine (ACh) at 1% or sodium nitroprusside (SNP) also at 1%. Both were diluted in NaCl 0.9% and prepared immediately before use. "
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    ABSTRACT: CADASIL (Cerebral Autosomal Dominant Arteriopathy with Subcortical Infarcts and Leukoencephalopathy) is caused by mutations in the Notch3 receptor expressed at the surface of vascular smooth muscle cells. The functional consequences of the disease at the peripheral microcirculation level are incompletely elucidated. In this study, we aimed to assess, in vivo, the endothelium-dependent and independent vasodilation of the skin microvasculature in CADASIL patients. Twenty-three affected subjects were compared with 23 gender and age-matched controls. The brachial artery endothelium-dependent and endothelium-independent vasodilation were assessed after forearm cuff occlusion and nitroglycerin administration. Skin vasoreactivity to transcutaneous administration of acetylcholine and sodium nitroprussiate, and after postocclusive hyperemia were measured by Laser Doppler flowmetry. The maximum changes in the diameter of the brachial artery after the cuff release or after nitroglycerin administration did not differ between patients and controls. With iontopheresis, only the peak value of the dose response was found decreased in normocholesterolemic patients after nitroprussiate administration. The postocclusive test revealed a large increase of the time to peak value and whole duration of the hyperemic response in CADASIL patients. The results of this study show that the skin vasoreactivity is altered in CADASIL. Particularly, the kinetics of reactive hyperemia after cuff occlusion is dramatically changed with a lengthened and delayed response. This characteristic pattern may be related to the specific ultrastructural modifications related to Notch3 gene mutations involving smooth muscle cells in the microvasculature.
    Journal of Cerebral Blood Flow & Metabolism 03/2007; 27(2):250-7. DOI:10.1038/sj.jcbfm.9600356 · 5.41 Impact Factor
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    • "Anvendelsen og faktorer, der har betydning for resultaterne, er beskrevet i Guidelines fra en standardiseringsgruppe under ESCD (Bircher et al, 1994). Der er senere beskrevet en mere avanceret metode (Laser Doppler perfusion imaging) til visualisering af blodgennemstrømningen i huden (Fullerton et al, 2002). "

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