New Biomarker Evidence of Oxidative DNA Damage in Whole Saliva From Clinically Healthy and Periodontally Diseased Individuals

Department of Periodontology, Nihon University School of Dentistry, Tokyo, Japan.
Journal of Periodontology (Impact Factor: 2.71). 05/2002; 73(5):551-4. DOI: 10.1902/jop.2002.73.5.551
Source: PubMed


There is an increasing body of evidence implicating reactive oxygen species in the pathogenesis of periodontal tissue destruction. 8-Hydroxy-deoxyguanosine (8-OHdG) is one of the most commonly used markers to evaluate oxidative damage in a number of disorders including chronic inflammatory diseases. The aim of the present study was to evaluate 8-OHdG levels in whole saliva of patients with periodontitis and to assess the changes after initial treatment.
Saliva samples were collected from 78 patients with untreated periodontitis and 17 healthy control subjects. Clinical parameters and levels of 8-OHdG were assessed first to establish a baseline and again after initial periodontal treatment from 15 patients. 8-OHdG levels were determined by enzyme-linked immunosorbent assay.
The mean value of 8-OHdG in the saliva of periodontally diseased subjects, 4.28 +/- 0.10 ng/ml, was significantly higher (P<0.01) than that of clinically healthy subjects (1.56 +/- 0.10 ng/ml). A significant decrease in salivary 8-OHdG was observed after therapy (P<0.01).
In the present study, we evaluated for the first time 8-OHdG levels in whole saliva of patients with periodontitis and assessed changes after initial periodontal treatment. Our study indicated that 8-OHdG levels in saliva appear to reflect the status of periodontal health.

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    • "8-hydroxydeoxyguanosine (8-OHdG) is an oxidized nucleoside that is excreted in the bodily fluids with DNA. Takane et al.113 have demonstrated that the mean 8-OHdG level in saliva is a useful marker to screen periodontal disease. The level of 8-OHdG can be also used as a prognostic indicator to monitor the progression of periodontal disease.56 "
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    ABSTRACT: Traditional clinical measurements such as probing pocket depth, bleeding on probing, clinical attachment loss; plaque index and radiographs used for periodontal diagnosis are often of limited usefulness as they are indicators of previous periodontal disease rather than present disease activity. A literature search was carried out to find out all the available tests that indicate periodontal disease markers in saliva. All major databases were searched to compile the information on published reports between 1999 and 2014. The list of biomarkers available to date is compiled and presented in a table format. Each biomarker is discussed separately based on the available evidence. Based on the evidence, it can be concluded that several sensitive salivary indicators of periodontitis are available to detect the presence, severity and response to treatment. Further studies are warranted to analyze the sensitivity and reliability of these indicators that might help in developing non-invasive tests that could help in the diagnosis of periodontal disease.
    07/2014; 6(4):95-103.
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    • "Faccioni et al. [36] have detected a DNA damage in buccal mucosa because of the nickel and cobalt released from the brackets and arch-wires. Takane et al. [37] reported that the metal ions released by orthodontic materials can be identified in saliva, blood, and urine, albeit not in toxic concentrations. In parallel with these studies, the lack of a significant change in salivary levels of 8-OHdG in the treatment process supports the idea that full metal brackets, tubes, and wires are not toxic so as to cause an oxidative DNA damage. "
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    ABSTRACT: Aim: The aim of this study was to examine the changes in the levels of interleukine-1 beta (IL-1 β ), tumor necrosis factor alpha (TNF- α ), malondialdehyde (MDA), nitric oxide (NO), and 8-hydroxydeoxyguanosine (8-OHdG) in saliva and IL-1 β , TNF- α , and NO in gingival crevicular fluid (GCF) samples of patients with fixed orthodontic appliances. Material and method: The subject population consisted of 50 volunteers who were in need of orthodontic treatment with fixed orthodontic appliances. GCF and saliva samples were obtained from all individuals before treatment, at 1st month of treatment and at 6th month of treatment. Periodontal clinical parameters were measured. Samples were investigated to detect IL-1 β , TNF- α , and 8-OHdG levels using ELISA method and NO and MDA levels using spectrophotometric method. Results: Since IL-1 β level detected in GCF at the 6th month of orthodontic treatment is statistically significant according to baseline (P < 0.05), all other biochemical parameters detected both in saliva and in GCF did not show any significant change at any measurement periods. Conclusion: Orthodontic tooth movement and orthodontic materials used in orthodontic treatment do not lead to a change above the physiological limits that is suggestive of oxidative damage in both GCF and saliva.
    Disease markers 04/2014; 2014:597892. DOI:10.1155/2014/597892 · 1.56 Impact Factor
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    • "In association with oral and dental diseases (especially periodontitis), changed oxidative stress markers in saliva were reported [2] [46] [51]. Being a noninvasive biofluid, saliva allows easy, noninvasive and safe sam- ISSN 0278-0240/13/$27.50 "
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    ABSTRACT: BACKGROUND: Salivary markers of oxidative stress and antioxidant status represent promising tool for the research of oral diseases. One of the criteria is the validation of these biomarkers from the perspective of the confounding and modifying factors. AIM: To examine the effect of circadian rhythm, tooth-brushing and ascorbic acid treatment on selected salivary markers of oxidative and carbonyl stress, and antioxidant status. SUBJECTS AND METHODS: Whole unstimulated saliva samples were collected from 19 healthy participants three times during a day, before and after tooth-brushing, and before and after the administration of vitamin C (250 mg). Advanced oxidation protein products (AOPP), thiobarbituric acid reactive substances (TBARS), advanced glycation end products (AGEs), ferric reducing antioxidant power (FRAP) and total antioxidant capacity (TAC) were measured. RESULTS: Salivary AGEs levels varied significantly during the day (p < 0.05) with the highest concentrations in the morning. FRAP levels varied during the day (p < 0.01) with the highest concentrations in the afternoon. Tooth-brushing decreased AGEs (p < 0.05) and TBARS levels (p < 0.01) and increased FRAP levels (p < 0.05). Single intake of vitamin C significantly decreased AGEs (p < 0.001) and increased both FRAP (p < 0.01) and TAC (p < 0.01) concentrations. CONCLUSION: Significant daily variations were observed in salivary AGEs and FRAP levels. Tooth-brushing and treatment with vitamin C decreased carbonyl stress and increased the antioxidant status. These results are important from the perspective of using saliva for the research of oral diseases.
    Disease markers 03/2013; 34(5). DOI:10.3233/DMA-130975 · 1.56 Impact Factor
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