Article

The GOLD domain, a novel protein module involved in Golgi function and secretion.

National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA.
Genome biology (impact factor: 6.63). 02/2002; 3(5):research0023. pp.research0023
Source: PubMed

ABSTRACT Members of the p24 (p24/gp25L/emp24/Erp) family of proteins have been shown to be critical components of the coated vesicles that are involved in the transportation of cargo molecules from the endoplasmic reticulum to the Golgi complex. The p24 proteins form hetero-oligomeric complexes and are believed to function as receptors for specific secretory cargo.
Using sensitive sequence-profile analysis methods, we identified a novel beta-strand-rich domain, the GOLD (Golgi dynamics) domain, in the p24 proteins and several other proteins with roles in Golgi dynamics and secretion. This domain is predicted to mediate diverse protein-protein interactions. Other than in the p24 proteins, the GOLD domain is always found combined with lipid- or membrane-association domains such as the pleckstrin homology (PH), Sec14p and FYVE domains.
The identification of the GOLD domain could aid in directed investigation of the role of the p24 proteins in the secretion process. The newly detected group of GOLD-domain proteins, which might simultaneously bind membranes and other proteins, point to the existence of a novel class of adaptors that could have a role in the assembly of membrane-associated complexes or in regulating assembly of cargo into membranous vesicles.

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Keywords

bind membranes
 
cargo molecules
 
detected group
 
diverse protein-protein interactions
 
endoplasmic reticulum
 
FYVE domains
 
GOLD domain
 
GOLD-domain proteins
 
Golgi complex
 
Golgi dynamics
 
membrane-associated complexes
 
membrane-association domains
 
novel beta-strand-rich domain
 
novel class
 
p24 proteins
 
p24 proteins form hetero-oligomeric complexes
 
pleckstrin homology
 
secretion process
 
sensitive sequence-profile analysis methods
 
specific secretory cargo
 

Vivek Anantharaman