Determination of irbesartan in the presence of hydrochlorothiazide by derivative spectrophotometry.
ABSTRACT A first-derivative spectrophotometric method for the determination of irbesartan (IRB) alone and in the presence of hydrochlorothiazide (HCT) is described. Measurements are made at the zero-crossing wavelength at 263.0 nm for IRB. The calibration graph was linear over the range 1.0-12.0 mg l(-1) of IRB, detection limit was 0.15 mg l(-1). HCT, in the presence of IRB was determined by direct spectrophotometric method at 317 nm. The calibration graph was linear over the range 2.0-50.0 mg l(-1) of HCT, detection limit was 0.25 mg l(-1). The proposed methods were successfully applied to the determinations of IRB and HCT in combined tablets.
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Article: Analysis of Sartans: A Review.[Show abstract] [Hide abstract]
ABSTRACT: The risk of cardiovascular diseases is closely related to hypertension, high cholesterol levels, and diabetes. When these risk factors appear together they are referred to as a metabolic syndrome. In the treatment of cardiovascular diseases, a combination of antihypertensive, hypolipemiant, and antidiabetic drugs is often applied. Diuretics (chlortalidone, hydrochlorothiazide, etc.) and angiotensin II receptors antagonist (sartans) are used to control hypertension, whereas statins (fluvastatin, simvastatin, etc.) are used to reduce cholesterol levels. This review is concerned with methods for the analysis of sartans in various matrices, such as pharmaceutical formulations, environmental and biological samples, and discusses the current status of stability studies of sartans . It also presents analytical methods for the simultaneous determination of sartans, diuretics, and statins. © 2013 Wiley Periodicals, Inc. and the American Pharmacists Association J Pharm Sci.Journal of Pharmaceutical Sciences 11/2013; · 3.13 Impact Factor
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ABSTRACT: BACKGROUND: The new combination of moxifloxacin HCl and cefixime trihydrate is approved for the treatments of lower respiratory tract infections in adults. At initial formulation development and screening stage a fast and reliable method for the dissolution and release testing of moxifloxacin and cefixime were highly desirable. The zero order overlaid UV spectra of moxifloxacin and cefixime showed >90% of spectra are overlapping. Hence, simple, accurate precise and validated two derivative spectrophotometric methods have been developed for the determination of moxifloxacin and cefixime. METHODS: In the first derivative spectrophotometric method varying concentration of moxifloxacin and cefixime were prepared and scanned in the range of 200 to 400 nm and first derivative spectra were calculated (n = 1). The zero crossing wavelengths 287 nm and 317.9 nm were selected for determination of moxifloxacin and cefixime, respectively. In the second method the first derivative of ratio spectra was calculated and used for the determination of moxifloxacin and cefixime by measuring the peak intensity at 359.3 nm and 269.6 nm respectively. RESULTS: Calibration graphs were established in the range of 1--16 mug /mL and 1--15 mug /mL for both the drugs by first and ratio first derivative spectroscopic methods respectively with good correlation coefficients. Average accuracy of assay of moxifloxacin and cefixime were found to be 100.68% and 98 93%, respectively. Relative standard deviations of both inter and intraday assays were less than 1.8%. Moreover, recovery of moxifloxacin and cefixime was more than 98.7% and 99.1%, respectively. CONCLUSIONS: The described derivative spectrophotometric methods are simple, rapid, accurate, precise and excellent alternative to sophisticated chromatographic techniques. Hence, the proposed methods can be used for the quality control of the cited drugs and can be extended for routine analysis of the drugs in formulations.Chemistry Central Journal 09/2012; 6(1):105. · 1.31 Impact Factor
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ABSTRACT: A simple matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) method was developed to analyze irbesartan in human plasma. Irbesartan is a kind of angiotensin II receptor blocker (ARB) and is used as an antihypertensive drug. MALDI-TOF MS is a rare application for clinical drug analysis in human plasma. After simple micro-liquid-liquid extraction, irbesartan-containing supernatant was spotted on a target plate, mixed with matrix and then detected by MALDI-TOF MS within the clinically therapeutic range. Furthermore, we used cheaper chemical analogues to label the major proteins in human plasma for protein quantitation. After enzyme digestion, peptide mixtures were injected into nanoliquid chromatography (nanoLC) coupled with tandem mass spectrometry (MS-MS). Protein identification could be carried out simultaneously by peptide sequencing and database searching. Chemical analogue labeling method is an alternative way for expensive isotope reagents. Quantity change of proteins before and after administration of irbesartan could be detected by this method. Application of these methods in human plasma demonstrated that these two micro-scale MS methods used for clinical drug monitoring, protein quantitation and identification are successful.Journal of pharmaceutical and biomedical analysis 01/2011; 54(1):100-5. · 2.45 Impact Factor