Determination of irbesartan in the presence of hydrochlorothiazide by derivative spectrophotometry
ABSTRACT A first-derivative spectrophotometric method for the determination of irbesartan (IRB) alone and in the presence of hydrochlorothiazide (HCT) is described. Measurements are made at the zero-crossing wavelength at 263.0 nm for IRB. The calibration graph was linear over the range 1.0-12.0 mg l(-1) of IRB, detection limit was 0.15 mg l(-1). HCT, in the presence of IRB was determined by direct spectrophotometric method at 317 nm. The calibration graph was linear over the range 2.0-50.0 mg l(-1) of HCT, detection limit was 0.25 mg l(-1). The proposed methods were successfully applied to the determinations of IRB and HCT in combined tablets.
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ABSTRACT: A simple and accurate method is presented for determination of irbesartan in pure form and commercial dosage forms. The method is based on the reaction of the above cited drug with bromophenol blue (BPB), bromocresol purple (BCP), bromothymol blue (BTB) and naphthol blue black (NBB) dyes in solutions containing Britton buffer to form ion-pair complexes extractable with chloroform and subsequently measured spectrophotometrically at 420nm for BPB, BCP, BTB and 625nm for NBB. All the reaction conditions for the proposed method have been studied. The reactions were extremely rapid at room temperature and the absorbance values remains unchanged up to 24hrs. Beer's law was obeyed in the ranges 1.8-45, 1.5-43, 2-60 and 10-244 g/mL for BPB, BCP, BTB and NBB, respectively. Interferences of the other ingredients and excipients were not observed. The proposed method is simple, fast and sensitive, and the first reported extractive method for the determination of irbesartan.
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ABSTRACT: A second-derivative spectrophotometric method for the simultaneous determination of irbesartan (IRB) and hydrochlorothiazide (HCT) in tablets is described. Measurements were made at the zero-crossing wavelengths at 230.1 nm for IRB and 232.7 nm for HCT. The calibration graphs were linear in the range of 14.4–33.6 mg L for IRB and 1.2–2.8 mg L for HCT. The limits of quantitation were 5.0 and 1.1 mg L. The proposed method was successfully applied to the simultaneous determination of IRB and HCT in commercial tablets with a high percentage of recovery, good accuracy, and precision.Analytical Letters 01/2003; No. 11(11-pp. 2485–2495):2485-2495. DOI:10.1081/AL-120024337 · 0.98 Impact Factor
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ABSTRACT: A differential pulse (DP) and square wave (SW) voltammetric techniques were developed for the determination of irbesartan. The electrochemical behavior of irbesartan was investigated by cyclic voltammetry (CV), differential pulse voltammetry (DPV), and square wave voltammetry (SWV) at the hanging mercury drop electrode (HMDE). Different parameters were tested to optimize the conditions of the determination. It was found that in the range of 8 × 10-6-1 × 10-4 M, the currents measured by both of methods presented a good linear property as a function of the concentration of irbesartan. In addition, validation parameters, such as reproducibility, sensitivity, and recovery were evaluated as well. The slope of the log Ip- log linear plot was 0.58 indicating the diffusion control for 0.5 M sulphuric acid without the need for separation or complex sample preparation, since there was no interference from the excipients and endogenous substances. The methods were successfully applied to the analysis of irbesartan in the pharmaceutical tablet formulations and in human serum samples.Analytical Letters 09/2009; 42(14):2322-2338. DOI:10.1080/00032710903137491 · 0.98 Impact Factor