Article

Intracellular trafficking of yeast telomerase components.

Swiss Institute for Experimental Cancer Research (ISREC), Epalinges, Switzerland.
EMBO Reports (Impact Factor: 7.86). 08/2002; 3(7):652-9. DOI: 10.1093/embo-reports/kvf133
Source: PubMed

ABSTRACT Telomerase uses an internal RNA moiety as template for the synthesis of telomere repeats. In Saccharomyces cerevisiae, the telomerase holoenzyme contains the telomerase reverse transcriptase subunit Est2p, the telomerase RNA moiety TLC1, the telomerase associated proteins Est1p and Est3p, and Sm proteins. Here we assess telomerase assembly by determining the localization of telomerase components. We found that Est1p, Est2p and TLC1 can migrate independently of each other to the nucleus. With limiting amounts of TLC1, overexpressed Est1p and Est2p accumulated in the nucleolus, whereas enzymatically active Est2p-TLC1 complexes are distributed over the entire nucleus. The distribution to the nucleoplasm depended on the specific interaction between Est2p and TLC1 but was independent of Est1p and Est3p. Altogether, our results suggest a role of the nucleolus in telomerase biogenesis. We also describe experiments that support a transient cytoplasmic localization of TLC1 RNA.

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Available from: Susan M Gasser, Jul 17, 2015
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    • "The nucleolus seems to be coupled with variety of telomeric functions, given that many telomere-related functions are known to localize in the nucleoli in Arabidopsis and other organisms. For example, telomerase localizes in the nucleoli during S phase in mammalian cells and yeast (Etheridge et al., 2002; Teixeira et al., 2002; Wong et al., 2002; Khurts et al., 2004; Yan et al., 2004) and the N-terminal part of AtTERT is localized in the nucleolus in Arabidopsis (Rossignol et al., 2007). The telomere-associated protein, Rap1, from yeast is a Myb-like protein with additional functions in transcription (Horvath, 2008). "
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    • "Telomerases differ in catalytic properties such as processivity and fidelity, and telomerases of some organisms function as dimers (Beattie et al., 2001; Wenz et al., 2001; reviewed in Ly et al., 2003; Autexier and Lue, 2006; Cohen et al., 2007). Biosynthesis of both subunits, TERT and TR, is not directly connected, and differential regulation raises questions of intracellular/intranuclear trafficking and their assembly to form an active telomerase (Teixeira et al., 2002; Tomlinson et al., 2006; for reviews see Collins, 2006; Hug and Lingner, 2006; Cairney and Keith, 2008; Gallardo et al., 2008). Generally, functional telomerase in vivo has many inter-related activities (Figure 1), including RNA–protein interactions (binding of TR and TERT subunits), DNA–protein interactions (binding to substrate), RT activity and protein–protein interactions (e.g. "
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    • "There is a great deal of divergence in maturation pathways among eukaryotic telomerase snRNPs, for example, ciliate telomerase does not contain Sm proteins (Collins 2006). Yeast telomerase snRNPs have been shown to be assembled in the cytoplasm and imported into the nucleus (Ferrezuelo et al. 2002; Teixeira et al. 2002), and in humans, telomerase components are found in Cajal bodies (Fu and Collins 2006), suggesting that maturation pathways in at least some metazoans are similar to those used by spliceosomal and U7 snRNPs. A wide variety of eukaryotes process mono-and/or polycistronic transcripts via trans-splicing, in which a fragment of a ''leader'' RNA is spliced onto each cistron from a specialized splice leader (SL) snRNA found within a specialized SL snRNP (Hastings 2005). "
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