Antitumor effects induced by dendritic cell-based immunotherapy against established pancreatic cancer in hamsters.
ABSTRACT Because the prognosis of patients with pancreatic cancer is very poor, development of a novel approach for treatment of this disease is vital. In the present study, we investigated the effect of dendritic cell (DC)-based immunotherapy against established syngeneic hamster pancreatic cancer named HPD1NR. Hamster enriched DCs were prepared from bone marrow (BM) by a culture for 7 days in the presence of mouse GM-CSF and mouse IL-4, and characterized by the expression of specific DC markers (DEC205, DC-SIGN) mRNA using in situ hybridization (ISH). DCs pulsed with tumor lysate and N-[1-(2,3-dioleoyloxy)propyl]-N,N,N-trimethylammonium methylsulfate (DOTAP) or DCs alone were injected s.c. weekly into HPD1NR-bearing hamsters three times. Tumor growth was significantly inhibited by 82% in hamsters treated with tumor lysate and DOTAP-pulsed DCs when compared with the PBS vehicle-treated group. These findings suggest that DC-based immunotherapy may be a useful approach for the treatment of pancreatic cancers.
- SourceAvailable from: europepmc.org
[Show abstract] [Hide abstract]
- "Immunotherapy studies using these cells have been published for melanoma, kidney, and prostate cancers. The first results for an animal model of pancreatic cancer were published in 2003 by Akiyama et al., who found tumor growth inhibition in 82% of mice . Later, Irisawa et al. performed EUS-guided injection of immature DCs in seven patients with prior chemotherapy (e.g., gemcitabine) failure . "
ABSTRACT: Since the development of endoscopic ultrasound-guided fine needle aspiration (EUS-FNA) in the early 1990s, its application has been extended to various diseases. For pancreatic cancer, EUS-FNA can obtain specimens from the tumor itself with fewer complications than other methods. Interventional EUS enables various therapeutic options: local ablation, brachytherapy, placement of fiducial markers for radiotherapy, and direct injection of antitumor agents into cancer. This paper will focus on EUS-guided oncologic therapy for pancreatic cancer.Diagnostic and Therapeutic Endoscopy 02/2013; 2013:157581. DOI:10.1155/2013/157581
Article: This month in APRArchives of Pharmacal Research 06/2010; 33(6):793-795. DOI:10.1007/s12272-010-0600-z · 1.75 Impact Factor