Article

Overexpression of a bacterial indole-3-acetyl-l-aspartic acid hydrolase in Arabidopsis thaliana.

Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst, MA 01003, USA.
Physiologia Plantarum (impact factor: 3.11). 09/2002; 115(4):513-522. pp.513-522
Source: PubMed

ABSTRACT Transgenic Arabidopsis lines (ecotype Col-0) carrying the Enterobacter agglomerans IaaspH gene under CaMV 35S promoter control were more sensitive to exogenous indole-3-acetyl aspartic acid (IAA-Asp) and metabolized [2'-14C]IAA-Asp more rapidly than control lines. Free IAA, total IAA and IAN levels in independent transgenic lines that accumulated IaaspH mRNA varied insignificantly from control levels, yet IAA-Asp levels were significantly reduced. The transgenic lines were grown in a variety of conditions and subjected to morphometric analysis. All three lines showed statistically significant differences in rosette diameter (in soil), root and hypocotyl length (on agar). These effects were transient in some cases and did not manifest themselves under all growth conditions tried. The two independent lines with single T-DNA insertions had lower seed set compared to control lines.

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    Article: A high-throughput method for the quantitative analysis of auxins.
    Lana S Barkawi, Yuen-Yee Tam, Julie A Tillman, Jennifer Normanly, Jerry D Cohen
    [show abstract] [hide abstract]
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    Nature Protocol 09/2010; 5(10):1609-18. · 8.36 Impact Factor
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Keywords

accumulated IaaspH mRNA varied insignificantly
 
CaMV 35S promoter control
 
cases
 
ecotype Col-0
 
Enterobacter agglomerans IaaspH gene
 
exogenous indole-3-acetyl aspartic acid
 
hypocotyl length
 
independent transgenic lines
 
metabolized [2'-14C]IAA-Asp
 
morphometric analysis
 
rosette diameter
 
single T-DNA insertions
 
statistically significant differences
 
Transgenic Arabidopsis lines
 
transgenic lines
 
two independent lines