Alpha-tocopherol transfer protein is specifically localized at the implantation site of pregnant mouse uterus.
ABSTRACT Alpha-tocopherol transfer protein (alpha-TTP) was first described to play a major role in maintaining alpha-tocopherol levels in plasma, while alpha-tocopherol was primarily reported to be a factor relevant for reproduction. Expression of alpha-TTP is not only seen in the liver, from where it was first isolated, but also in mouse uterus, depending on its state of pregnancy, stressing the importance of alpha-TTP for embryogenesis and fetal development. The cellular localization of alpha-TTP in mouse uterus is reported here. By immunohistochemistry, alpha-TTP could be localized in the secretory columnar epithelial cells of the pregnant uterus on Days 4.5 and 6.5 postcoitum as well as in the glandular epithelial cells and the inner decidual reaction zone surrounding the implantation site. On Days 8.5 and 10.5 postcoitum (midterm of mouse pregnancy), alpha-TTP could still be detected in the uterine secretory columnar epithelial cells, while in alpha-TTP knockout mice, no immunostaining was visible. It is suggested that alpha-TTP plays a major role in supplying the placenta and consecutively the fetus with alpha-tocopherol throughout pregnancy. We conclude that alpha-tocopherol plays a role in the process of implantation and that alpha-TTP may be necessary for adequate alpha-tocopherol status of the fetus.
SourceAvailable from: Yusof Kamisah[Show abstract] [Hide abstract]
ABSTRACT: Introduction: Hyperbilirubinaemia (jaundice) is common during the first days of postnatal life. α-Tocopherol was reported to decrease serum total bilirubin in jaundiced human newborns. This study was conducted to investigate the effect of maternal palmvitee administration on hyperbilirubinaemia induced by δ-aminolevulinic acid (ALA) in rat neonates. Material and methods: Twenty-four successfully mated female Wistar rats were divided into three groups. They were given 50 or 200 mg palmvitee/kg body weight orally once daily from day 1 of pregnancy to delivery, while the other group was given olive oil (control). At postnatal day 14 (the optimal age to induce hyperbilirubinaemia as obtained earlier), the pups born to four dams of each group were induced with hyperbilirubinaemia, while the rest were given vehicle. Twenty-four hours after the induction, the neonates were sacrificed. Plasma total bilirubin, hepatic thiobarbituric acid reactive substance (TBARS), uridine diphosphate glucuronyltransferase (UGT) activity and vitamin E content were determined in the neonates. Results: ALA administration increased plasma total bilirubin. In the palmvitee-treated groups, plasma total bilirubin was lower than in the controls (0.19±0.01 and 0.10±0.01 vs. 0.35±0.18, p<0.05). ALA did not affect the hepatic UGT, but it was reduced in the palmvitee-treated groups (0.71±0.10 and 0.55±0.02 vs. 0.92±0.07, p<0.05). Neither ALA nor palmvitee influenced hepatic TBARS level. Maternal pretreatment with 200 mg/kg palmvitee increased the neonatal hepatic vitamin E content. Conclusions: The maternal administration of palmvitee showed a protective effect on hyperbilirubinaemia. However, this administration could lead to decreased hepatic glucuronidation activity in rat neonates.Archives of Medical Science 03/2008; 4(1):32-39. · 1.89 Impact Factor
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ABSTRACT: The α-tocopherol transfer protein (α-TTP) is a ~32kDa cytosolic protein that plays an important role in the efficient circulation of plasma α-tocopherol in the body, a factor with great relevance in reproduction. The α-TTP gene has been studied in a number of tissues; however, its expression and function in some ovine tissues remain unclear. A previous study from our laboratory has demonstrated α-TTP expression in sheep liver. In the present study we determined whether α-TTP is expressed in non-liver tissues and investigated the effects of dietary vitamin E on the α-TTP mRNA levels. Thirty-five male Tan sheep with similar body weight were randomly allocated into five groups and supplemented 0, 20, 100, 200 and 2000IUsheep(-1)day(-1) vitamin E, for four months, respectively. At the end of the study, the animals were slaughtered and tissue samples from the heart, spleen, lung, kidney, longissimus dorsi muscle and gluteus muscle were immediately collected. We found that the α-TTP gene is expressed in sheep tissues other than the liver. Moreover, dietary vitamin E levels had influenced the expression levels of α-TTP gene in these tissues in a tissue-specific way. The technique of immunohistochemistry was used to detect α-TTP in tissues of the heart, spleen, lung, and kidney and we found that α-TTP was mainly located in the cytoplasm while no α-TTP immunoreactivity was detected in the cytoplasm of longissimus dorsi and gluteus muscle samples. Importantly, our findings lay the foundation for additional experiments focusing on the absorption and metabolism of vitamin E in tissues other than the liver.Gene 03/2014; DOI:10.1016/j.gene.2014.03.014 · 2.08 Impact Factor