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Developmental and spatial expression pattern of syntaxin 13 in the mouse central nervous system.

Institut de Biologie Cellulaire et de Morphologie (IBCM), Rue du Bugnon 9, 1005 Lausanne, Switzerland.
Cell and Tissue Research (Impact Factor: 3.33). 09/2002; 309(2):209-18. DOI: 10.1007/s00441-002-0600-5
Source: PubMed

ABSTRACT Vesicular transport involves SNARE (soluble- N-ethylmaleimide-sensitive-factor-attachment-protein-receptor) proteins on transport vesicles and on target membranes. Syntaxin 13 is a SNARE enriched in brain, associated with recycling endosomes; its overexpression in PC12 cells promotes neurite outgrowth. This suggests an important role for receptor recycling during neuronal differentiation. Here we describe the spatiotemporal pattern of syntaxin 13 expression during mouse brain development. During early embryogenesis (E12-E15), it was found in the forebrain ventricular zone and in primary motor and sensory neurons in the brainstem, spinal cord and sensory ganglia. In the forebrain at E15, syntaxin 13 was not detected in neuroblasts in the intermediate zone of the embryonic hemispheric wall, while there was labeling in cortical neurons in deeper layers starting at E15-18, and progressively in later-generated neurons up to layer II around P6. Syntaxin 13 reached maximal expression in all brain divisions at about P7, followed by a decrease, with heterogeneous neuron populations displaying various staining intensities in adult brain. While usually restricted to the soma of neurons, we transiently detected syntaxin 13 in dendrites of pyramidal neurons during the first postnatal week. In conclusion, the developmentally regulated syntaxin 13 expression in various neuronal populations is consistent with its involvement in endocytic trafficking and neurite outgrowth.

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    • "In addition, its expression is enriched in brain and overexpression enhances neurite outgrowth in NGF-stimulated PC12 cells [Hirling et al., 2000]. Interestingly, syntaxin 13 and the sr-PLPs share a striking similarity in their subcellular localization as well as in both their spatial and temporal patterns of expression during development [Sarria, 2002]. The similarities in expression profile suggest that the sr-isoforms may play a role in vesicular transport in neurons comparable to that identified in OLs [Bongarzone et al., 2001]. "
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    • "This finding is probably due to a substitution of syntaxin 3 by another syntaxin isoform. A possible candidate is syntaxin 13, which was shown to be important for axon elongation and growth cone expansion in other systems (Hirling et al. 2000; Sarria et al. 2002). Late ontogenetic expression of syntaxin has also been reported in the pineal gland, which is a retina-related tissue (Redecker 2000). "
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