Quantitation of platelet adhesion to Ti and DLC-coated Ti in vitro using (125)I-labeled platelets.

L K Krishnan, N Varghese, C V Muraleedharan, G S Bhuvaneshwar, F Derangère, Y Sampeur, R Suryanarayanan

BMT Wing, Si Chitra Tirunal Institute of Medical Sciences, Thiruvanandhapuram 690512, India.

Journal Article: Biomolecular Engineering (impact factor: 3.17). 09/2002; 19(2-6):251-3.

Abstract

Measurement of platelet adhesion in vitro is a good indicator of its reactivity to implant devices in vivo. Platelets were labeled with I-125 without affecting its normal morphology and function and the labeled platelets were suspended in platelet poor plasma and exposed to Ti and diamond like carbon-coated (DLC) Ti discs, under dynamic conditions, using a parallel plate flow chamber. The test materials were washed, dried, exposed to a phosphor screen and scanned to get the images. The number of platelets that adhered to Ti was found to be higher than those that adhered to DLC coated Ti sample, irrespective of the shear stress which was varied between 2 and 16 dynes/cm(2).

Source: PubMed

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Keywords

adhered
 
dynamic conditions
 
good indicator
 
implant devices
 
labeled platelets
 
normal morphology
 
parallel plate flow chamber
 
platelet adhesion
 
platelet poor plasma
 
platelets
 
scanned
 
shear stress
 
test materials