Article

Zymographic detection of cinnamic acid decarboxylase activity.

Department of Microbiology, Faculty of Biology, University of Barcelona, Avenue Diagonal 645, 08028 Barcelona, Spain.
Journal of Microbiological Methods (impact factor: 2.09). 12/2002; 51(3):417-20. pp.417-20
Source: PubMed

ABSTRACT The manuscript includes a concise description of a new, fast and simple method for detection of cinnamic acid decarboxylase activity. The method is based on a color shift caused a by pH change and may be an excellent procedure for large screenings of samples from natural sources, as it involves no complex sample processing or purification. The method developed can be used in preliminary approaches to biotransformation processes involving detection of hydroxycinnamic acid decarboxylase activity.

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Keywords

biotransformation processes
 
cinnamic acid decarboxylase activity
 
color shift
 
complex sample processing
 
concise description
 
excellent procedure
 
fast
 
hydroxycinnamic acid decarboxylase activity
 
large screenings
 
natural sources
 
preliminary approaches