Lipid Peroxidation and Antioxidant Enzymes in Turkish Population: Relation to Age, Gender, Exercise, and Smoking.
The purpose of this study was to examine the change in lipid peroxidation and antioxidant enzyme activities in healthy subjects and to evaluate the concentrations of superoxide dismutase, glutathione peroxidase and malondialdehyde, an end product of lipid peroxidation in exercise and smoking. Study included 257 appearently healthy individuals, 133 males and 124 females. In all subjects, malondialdehyde (MDA) levels were analyzed as an indicator of the lipid peroxidation activities. Superoxide dismutase, glutathione peroxidase activities were measured as an indicator of antioxidant activities. Oxidative stress was estimated by the method based on thiobarbituric acid reactivity. Erythrocyte superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities were estimated on hemolysates by use of commercial available kits (Randox lab., Dublin, Ireland). For all groups serum lipid peroxidation and erythrocyte SOD and GSH-Px were obtained at the initial and the following periods. Serum MDA level was higher in the elderly than in the children and in the adults. MDA levels were higher in the smoking, acute exercise than their counterparts in the control groups. GSH-Px activity was significantly lower in the acute exercise group, and higher in the trained group than those as controls. SOD decreased in the elderly, smoking and acute exercise groups and increased in trained individuals. There was a significant increase in lipid peroxidation activity and a significant decrease in antioxidant enzyme activity in cases of acute exercise and smoking as well as the elderly.
- "Among biological compounds, lipids, especially PUFAs, are the most susceptible targets for free radicals induced damages. An imbalance between the concentration of oxidative species and the cell antioxidant system leads to the cell membrane oxidative damage, due to the high content of lipids, particularly PUFAs (Ozbay & Dülger, 2002). In the present study, the level of total PUFAs was significantly increased (P < 0.05) after 3 months of chokeberry juice consumption. "
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ABSTRACT: The effect of long-term polyphenol-rich chokeberry juice consumption on activities of antioxidant enzymes and membrane lipid status in erythrocytes of 25 healthy women was examined. Percentages of membrane fatty acids, superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities were assessed at a baseline, in the middle and at the end of a 3-month-long consumption period. A significant increase in C22:6n-3, n-3 polyunsaturated fatty acids (PUFAs), total PUFAs and unsaturation index and a significant decrease in monounsaturated fatty acids (MUFAs) and n-6:n-3 ratio were found. Significantly higher SOD and GPX activities were also recorded at the end of the study. Serum lipids and glucose were stabile during the consumption period, while the levels of thiobarbituric acid–reactive substances (TBARS), as serum indicator of lipid peroxidation, were reduced significantly. These results indicate a positive impact of regular chokeberry juice consumption on cellular oxidative damage and suggest its putative role in the protection against oxidative stress.
Journal of Functional Foods 07/2014; 9(1):89–97. DOI:10.1016/j.jff.2014.04.019 · 3.57 Impact Factor
Available from: Hasan Ekim
- "Under normal physiological conditions, the antioxidants are responsible for cellular protection against oxidative stress, namely, by the free radical scavenger enzyme superoxide dismutase (SOD), catalase, and glutathione peroxidase (GSH-Px) , thereby maintaining the vasorelaxant and antithrombotic effects of nitric oxide (NO) in the vasculature . These scavengers are strategically compartmentalized in subcellular organelles within the cell to provide maximum protection . The glutathione peroxidases (GSH-Px) are a family of enzymes which reduce oxidized lipids to their nontoxic metabolites and may thereby decrease vascular injury. "
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ABSTRACT: Venous thromboembolism has multifactorial origin and occurs in the context of complex interactions between environmental and genetic predisposing factors. Oxidative stress plays an important role in the physiopathology of venous thrombosis. Current study examined the role of oxidative stress and asymmetric dimethylarginine in the development of DVT with the parameters such as serum malondialdehyde (MDA), glutathione peroxidase (GSH-Px), catalase, ADMA, homocysteine, folic acid, vitamin B6, and vitamin B12 levels. Serum MDA levels were found significantly (P < 0.005) high in patients with DVT compared with control group. Additionally, serum B6 levels were found significantly (P < 0.009) low in patients with DVT compared with healthy volunteers. There were no significant differences between the groups in terms of the other parameters (P > 0.05). This study showed that patients with DVT have increased oxidative stress compared with the healthy volunteers whereas there was no significant difference between the groups in terms of serum ADMA levels. Thus serum ADMA levels seemed to be not related with development of DVT.
Biochemistry Research International 04/2014; 2014:703128. DOI:10.1155/2014/703128
Available from: Inmaculada Tasset
- "Central to this defense are antioxidant enzymes, which include SOD and GPx (Franco et al. 2007). A study conducted in a Turkish population established that age, gender, and physical exercise are associated with SOD and GPx (Ozbay and Dülger 2002). That study found no gender-based differences in any antioxidant enzyme; however, there were higher levels of SOD and GPx in children, adolescents, and adults than in elderly people after acute exercise, and lower levels of SOD and GPx in adults. "
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ABSTRACT: Tissue damage resulting from oxidative stress induced by a pathological condition might have more serious consequences in children than in adults. Researchers have not yet identified particular markers - alone or in combination with others - of oxidative stress, or their role in pediatric diseases. The aim of this study was to identify gender-based biomarkers for measuring oxidative stress. Oxidative biomarkers were studied in 138 healthy Spanish children (85 boys, 53 girls) 7 to 12 years of age, at the prepubertal (Tanner I) stage, independent of body mass index (BMI), age, fitness (measured by 20-m shuttle run test), and physical activity (measured by participation in an after-school exercise program). The oxidative biomarkers measured were lipid peroxidation products, total nitrites, protein carbonyls, and oxidized glutathione (GSSG). The antioxidant biomarkers measured were total glutathione (TG), reduced glutathione (GSH), superoxide dismutase activity (SOD), and glutathione peroxidase activity. In the study population, height, weight, waist circumference, and BMI were lower in girls than in boys. For oxidative biomarkers, boys had higher levels of protein carbonyl than girls (p < 0.001). In spite of this, girls had higher levels of GSSG (p < 0.001) and TG (p = 0.001), and a lower GSH/GSSG ratio (p < 0.001) than boys. For the antioxidant response, girls had higher levels of SOD (p = 0.002) than boys. All analyses were adjusted for BMI, age, fitness, and physical activity. In conclusion, prepubertal girls had higher oxidative stress than boys, in addition to higher levels of SOD, independent of age, BMI, fitness, and physical activity.
Applied Physiology Nutrition and Metabolism 04/2013; 38(4):421-426. DOI:10.1139/apnm-2012-0219 · 2.34 Impact Factor
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