Toxic contaminants and their biological effects in coastal waters of Xiamen, China. II. Biomarkers and embryo malformation rates as indicators of pollution stress in fish.

Australian Institute of Marine Science, Townsville MC, Qld.
Marine Pollution Bulletin (Impact Factor: 2.99). 09/2002; 44(8):761-9.
Source: PubMed


Baseline information is presented on embryo malformation rate and biomarkers in fish as indicators of sub-lethal stress caused by pollution in coastal waters of Xiamen, PR China. Fish and eggs were sampled from several areas in Xiamen coastal waters (Xiamen Harbour, Maluan and Tongan Bays and East Channel), where varying levels of pollutant input have been documented. Comparative sampling was done at a "cleaner" reference site at Dongshan Island. Embryonic malformation rates, which indicate general water quality, varied with location and species of fish, and exceeded background levels for unpolluted waters (assumed approximately 5%) by up to eightfold at some sites. Generally, sites around Xiamen Harbour show signs of poor water quality having highest mean levels of embryo deformity (20-30%) and these decreased towards open waters (Tongan Bay, Eastern Channel) where abnormalities approached background levels. An indication that toxic contaminants may be having a localised effect in the region, particularly in the harbour was reinforced by the biomarker assays. However, activities of the biomarkers ethoxyresorufin O-deethylase (EROD) and glutathione S-transferase in fish livers indicate no clear pattern, and there is no evidence that fish from the four sampling areas have been more or less exposed to PAHs and other compounds that induce these biomarkers. Antioxidant biomarkers (glutathione peroxidase, catalase, superoxide dismutase, and reduced glutathione) suggest that exposure to xenobiotics appears to be lowest in Dongshan and Maluan and highest in the harbour and Tongan. Inhibition of acetylcholinesterase in fish muscle indicated possible effects by organophosphate and carbamate pesticides in Xiamen waters and these effects may be greatest in the area of the harbour.

37 Reads
  • Source
    • "Interestingly, another study of Coho salmon indicated that measuring MT mRNA levels in the olfactory system of salmon was more sensitive than in the liver, suggesting tissue‐specific differences in MT induction (Espinoza et al. 2012). Acetylcholinesterase (AChE) inhibition has long been used as a biomarker for exposure to, as well as effects of, carbamate and organophosphorus pesticides in both fish and invertebrates (reviewed in Fulton and Key 2001; Klumpp et al. 2002), although it is not always as effective a biomarker for mollusks due to avoidance behaviors (Cooper and Bidwell 2006). AChE inhibition is a rare and in some ways ideal biomarker as it indicates both exposure and effects. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Ensuring the health of aquatic ecosystems and identifying species at risk from the detrimental effects of environmental contaminants can be facilitated by integrating analytical chemical analysis with carefully selected biological endpoints measured in tissues of species of concern. These biological endpoints include molecular, biochemical and physiological markers (i.e. biomarkers) that when integrated, can clarify issues of contaminant bioavailability, bioaccumulation and ecological effects while enabling a better understanding of the effects of non-chemical stressors. In the case of contaminant stressors, an understanding of chemical modes of toxicity can be incorporated with diagnostic markers of aquatic animal physiology to help understand the health status of aquatic organisms in the field. Furthermore, new approaches in functional genomics and bioinformatics can help discriminate individual chemicals, or groups of chemicals among complex mixtures that may contribute to adverse biological effects. While the use of biomarkers is not a new paradigm, such approaches have been underutilized in the context of ecological risk assessment and natural resource damage assessment. From a regulatory standpoint, these approaches can help better assess the complex effects from coastal development activities to assessing ecosystem integrity pre- and post-development or site remediation. Integr Environ Assess Manag © 2014 SETAC.
    Integrated Environmental Assessment and Management 07/2014; 10(3). DOI:10.1002/ieam.1530 · 1.38 Impact Factor
  • Source
    • "Similar abnormalities has been reported (in different degrees) among others batoids species, such as Bathyraja richardsoni (Garrick, 1961) (Forster, 1967), Amblyraja radiata (Donovan, 1808), Raja brachyura Lafont, 1873 (Templeman, 1965), Potamotrygon motoro (Müller & Henle, 1841) (Rosas et al., 1996), Dasyatis akajei (Müller & Henle, 1841) (Honma & Sugihara, 1971) and Dasyatis longa (Garman, 1880) (Escobar-Sánchez et al., 2009). Congenital abnormalities are usually linked to different causes as: endogamy (Sari et al., 2008), parasites (Kelly et al., 2009) or with exposure to environmental degradation or pollution of the mother (Klumpp et al., 2002), unfortunately this study cannot be conclusive about the causes of this specifically malformation and further studies should be done on this and other species to identify their possible causes. "
    Cahiers de Biologie Marine 01/2011; 52:357-360. · 0.80 Impact Factor
  • Source
    • "In fish, developmental malformations have been linked to the presence of several environmental pollutants such as persistent organochlorines, pesticides, or heavy metals (Westernhagen von 1988). In several studies, direct embryotoxicity has resulted from the presence of complex matrices such as oil (Heintz et al. 1999), and recently, tests for embryonic malformations in fish have been used as general water quality indicators (Klumpp et al. 2002). "
    [Show abstract] [Hide abstract]
    ABSTRACT: Complex factors have contributed to the decline of aquatic populations worldwide. Among these factors are intensification of agriculture, including the application of fertilizers and agents of crop protection, and loss of habitat. Various developmental abnormalities in natural populations of aquatic vertebrates have been documented, and agricultural pesticides are considered by many to be one of the important factors that cause such abnormalities. Amphibians may potentially be a target of environmental stressors and toxicants as a result of their biphasic life cycles and skin permeability. In this chapter, the role of oxidative stress in the teratogenic action of pesticides is reviewed and addressed, with special attention given to non-target aquatic organisms such as amphibians, fish, and invertebrates. The review of available literature indicates that many pesticides enhance oxidative stress in aquatic organisms, and such stress may be linked to developmental alterations, including reproductive effects, embryotoxicity, and/or teratogenicity.
    Reviews of environmental contamination and toxicology 01/2011; 211:25-61. DOI:10.1007/978-1-4419-8011-3_2 · 3.74 Impact Factor
Show more