Article

When reverse genetics meets physiology: the use of site-specific recombinases in mice.

CNRS FRE2401, Molecular Genetics, Neurophysiology and Behavior, Institute of Biology, Collège de France, 11 place Marcelin Berthelot, 75231 Cedex 5, Paris, France.
FEBS Letters (Impact Factor: 3.34). 11/2002; 529(1):116-21. DOI: 10.1016/S0014-5793(02)03266-0
Source: PubMed

ABSTRACT The use of site-specific recombinases enables the precise introduction of defined genetic mutations into the mouse genome. In theory, any deletion, point mutation, inversion or translocation can be modeled in mice. Because gene targeting is controlled both spatially and temporally, the function of a given gene can be studied in the desired cell types and at a specific time point. This 'genetic dissection' allows to define gene function in development, physiology or behavior. In this review, we focus on the technical possibilities of Cre and other site-specific recombinases but also discuss their limitations.

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