IL-12 enhances the natural killer cell cytokine response to Ab-coated tumor cells. J Clin Invest

Department of Molecular Virology, Immunology, and Medical Genetics, The Arthur G. James Comprehensive Cancer Center, The Ohio State University College of Medicine, Columbus, Ohio 43210, USA.
Journal of Clinical Investigation (Impact Factor: 13.77). 11/2002; 110(7):983-92. DOI: 10.1172/JCI15950
Source: PubMed

ABSTRACT The anti-tumor activity of recombinant mAb's directed against tumor cell growth receptors has generally been considered to result from direct antiproliferative effects, the induction of apoptosis, or possibly Ab-dependent cellular cytotoxicity mediated against tumor targets. However, it remains unclear to what degree these mechanisms actually aid in the clearance of Ab-coated tumor cells in vivo. We show here that NK cells secrete a distinct profile of potent immunostimulatory cytokines in response to dual stimulation with Ab-coated tumor cells and IL-12. This response could not be duplicated by costimulation with other ILs and was significantly enhanced in the presence of monocytes. Cytokine production was dependent upon synergistic signals mediated by the activating receptor for the Fc portion of IgG (FcgammaRIII) and the IL-12 receptor expressed on NK cells. Coadministration of Ab-coated tumor cells and IL-12 to BALB/c mice resulted in enhanced circulating levels of NK cell-derived cytokines with the capacity to augment anti-tumor immunity. These findings suggest that, in addition to mediating cellular cytotoxicity and apoptosis, the anti-tumor activity of mAb's might also result from activation of a potent cytokine secretion program within immune effectors capable of recognizing mAb-coated targets.

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    • "In vitro, this process is termed as ADCC. Laboratory studies have shown that HER2-overexpressing breast cancer cell lines are susceptible to ADCC in the presence of trastuzumab (Carson et al, 2001; Parihar et al, 2002; Gennari et al, 2004), and in vivo activity of trastuzumab has been correlated with significantly increased number of peritumoral lymphocytes and monocytes and in vitro ADCC (Gennari et al, 2004; Arnould et al, 2006). Additional studies have indicated that NK cells are keys for trastuzumab-mediated ADCC (Cooley et al, 1999; Clynes et al, 2000; Mimura et al, 2005). "
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    • "immunity, suggesting an additional role for CD16 [18] [19]. Moreover , various in vitro studies showed that NK activated through the CD16 receptor produce TNF-a and INF-c which is potentiated when both IL-12 receptor and CD16 were mutually engaged [20] [21]. In the present study we present evidences demonstrating that cross-linking of CD16 on NK cells from human peripheral blood, up-regulates the expression of activating markers and receptors such as CD25, CD69, CD40L, NKp44, NKp30 and the intensity of CD56 expression. "
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    ABSTRACT: Human NK cells are classified into two populations according to the intensity of CD56 surface expression, as well as possession of CD16, FcRIII. CD56(dim)CD16(bright) make up 90% circulating NK cells, whereas CD56(bright)CD16(-/dim) comprises the remaining 10%. Here we report that peripheral NK cells upon CD16 cross-linking up-regulates the expression of activating markers and receptors such as CD25, CD69, NKp44, NKp30, CD40L and the intensity of CD56 expression. Additionally, co-culturing immature DCs with CD16 activated NK cells was found to significantly increase the expression of maturation markers on DCs. These results suggest that CD16 cross-linking on resting peripheral blood NK cells triggers the activation of these cells and induces the appearance of CD56(bright) NK cells. The latter were found capable of producing pro-inflammatory cytokines, IFN-gamma and TNF-alpha and notably IL-12.
    Cellular Immunology 01/2010; 264(1):86-92. DOI:10.1016/j.cellimm.2010.05.002 · 1.87 Impact Factor
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    • "Note that the highest antitumor activity has been found when Trastuzumab is combined with paclitaxel (Burstein , 2003), which suppresses adaptive immunity but selectively increases NK activity (Tsavaris et al., 2002). On the other hand, efforts to stimulate NK activity through specific cytokines (Parihar et al., 2002; Repka et al., 2003 "
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