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Matrix metalloproteinase-2, membranous type 1 matrix metalloproteinase, and tissue inhibitor of metalloproteinase-2 expression in ectopic and eutopic endometrium

Department of Obstetrics and Gynecology, Stanford University, Palo Alto, California, United States
Fertility and Sterility (Impact Factor: 4.59). 11/2002; 78(4):787-95. DOI: 10.1016/S0015-0282(02)03322-8
Source: PubMed

ABSTRACT To investigate expression of matrix metalloproteinase-2 (MMP-2), membranous type 1 matrix metalloproteinase (MT1-MMP), and tissue inhibitor of metalloproteinase-2 (TIMP-2) in ectopic and eutopic endometrium from women with and without endometriosis throughout the menstrual cycle.
Molecular studies in human tissue.
Reproductive immunology laboratory of a university medical center.
Fifty-three premenopausal woman (23 with endometriosis and 30 without endometriosis) undergoing laparoscopic surgery. Endometrium and ectopic endometriosis tissue were obtained at the time of surgery.
Messenger RNA and protein expression from eutopic and ectopic endometrium was analyzed by using quantitative competitive polymerase chain reaction, zymography, and Western blot assay.
Uterine endometrium from women with endometriosis expressed higher levels of MMP-2 and MT1-MMP and lower levels of TIMP-2 than did endometrium from normal women.
Eutopic endometrium from patients with endometriosis may be more invasive and prone to peritoneal implantation because of greater expression of MMP-2 and MT1-MMP and lower expression of TIMP-2 messenger RNA, compared with endometrium from women without endometriosis. Thus, increased proteolytic activity may help to explain the invasive factors that result in endometriosis.

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    • "Recent studies have underlined the critical role of MMP-2 in endometriosis [5]. MMP-2 belongs to the MMP family protein; these proteins play important roles in the processes of migration and invasion [18]. "
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    • "We then analyzed the expression of MMP2 and MMP9, which have been found to be up-regulated in active endometriotic lesions and may contribute to the invasive capacity of endometrial implants as well as to angiogenesis [41], [42], [43]. As shown in Figure 6, A and B, treatment of mice with ISO-1 had a significant downregulatory effect on the expression of MMP2 and MMP9 (P<0.05). "
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    • "Other factors have been shown to stimulate EPC recruitments such as stromal cell-derived factor-1, placental growth factor, and matrix metalloprotease-9.54–56 Interestingly, they all have been associated with endometriosis.57–60 "
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