Article

Alternate splicing in the cytosolic II-III loop and the carboxy terminus of human E-type voltage-gated Ca(2+) channels: electrophysiological characterization of isoforms.

Institute of Neurophysiology, University of Cologne, Robert-Koch-Strasse 39, D-50931 Cologne, Germany.
Molecular and Cellular Neuroscience (impact factor: 3.66). 11/2002; 21(2):352-65.
Source: PubMed

ABSTRACT There is growing evidence that Ca(v)2.3 (alpha1E, E-type) transcripts may encode the ion-conducting subunit of a subclass of R-type Ca(2+) channels, a heterogeneous group of channels by definition resistant to blockers of L-, N-, and P/Q-type Ca(2+) channels. To understand whether splice variation of Ca(v)2.3 contributes to the divergence of R-type channels, individual variants of Ca(v)2.3 were constructed and expressed in HEK-293 cells. With Ba(2+) as charge carrier, the tested biophysical properties were similar. In Ca(2+), the inactivation time course was slower and the recovery from short-term inactivation was faster; however, this occurred only in variants containing a 19-amino-acid-long insertion, which is typical for neuronal Ca(v)2.3 Ca(2+) channel subunits. This different Ca(2+) sensitivity is not responsible for the major differences between various R-type channels, and future studies might clarify its importance for in vivo synaptic or dendritic integration and the reasons for its loss in endocrine Ca(v)2.3 splice variants.

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Keywords

alpha1E
 
charge carrier
 
definition resistant
 
dendritic integration
 
endocrine Ca(v)2.3 splice variants
 
heterogeneous group
 
inactivation time course
 
individual variants
 
ion-conducting subunit
 
L-
 
major differences
 
splice variation
 
tested biophysical properties
 
typical