Article

Expression and clinical implication of soluble resistance-associated calcium-binding protein gene and multi-drug resistance gene in leukemia.

National Laboratory of Experimental Hematology, Hematologic Institute, Peking Union Medical College, Chinese Academy of Medical Sciences, Tianjin 300020, China.
Zhonghua zhong liu za zhi [Chinese journal of oncology] 07/2002; 24(4):370-4. pp.370-4
Source: PubMed

ABSTRACT To study the relationship between soluble resistance-related calcium-binding protein (sorcin) gene and multidrug resistance gene (mdr1), and their significance in clinical drug resistance and prognosis of acute myeloid leukemia (AML).
Amplification of sorcin gene and mdr1 gene in K562/A02 cell detected by Northern blot, were monitored by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) in 65 AML patients and 27 normal controls, with their relationship and clinical outcame analyzed.
The amplification of sorcin gene and mdr1 gene in AML patients were significantly higher than that in the normal control, which were related to clinical drug resistance and prognosis. The amplification of sorcin gene was related to the amplification of mdr1 gene in the two groups. The clinical drug resistance incidence rate and complete remission rate were 92.9% and 7.1% in sorcin(+)/mdr1(+) group. They were 8.6% and 91.4% in the sorcin(-)/mdr1(-) group (P < 0.001).
The co-amplification of sorcin and mdr1 gene can be taken as a good indicator of clinical drug resistance and prognosis of AML.

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Keywords

27 normal controls
 
65 AML patients
 
acute myeloid leukemia
 
AML
 
AML patients
 
amplification
 
clinical drug resistance
 
clinical drug resistance incidence rate
 
clinical outcame analyzed
 
co-amplification
 
complete remission rate
 
mdr1
 
mdr1 gene
 
multidrug resistance gene
 
normal control
 
Northern blot
 
semi-quantitative reverse transcription polymerase chain reaction
 
soluble resistance-related calcium-binding protein
 
two groups