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Available from: Christoph J Binder, Jul 15, 2014
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    • "Thirty-one patients and twelve healthy controls participated in this study with a 4-month follow up. Development of atherosclerosis was affected by the adaptive immune system (Binder et al., 2002) and Th1 are an important class of immune cells in the pathogenesis of atherosclerosis. Expression of vascular cell-adhesion molecule 1 (VCAM1) on endothelium in response to bioactive lipids such as ox-LDL that have been trapped within the arterial wall, is an early sign of the immune response to begin the process of atherosclerosis (Cybulsky & Gimbrone, 1991; Dai et al., 2004; Nakashima et al., 1998). "
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    ABSTRACT: Background: The aim of present study is evaluation of vitamin A supplementation efficacy on IFN-ɣ and T-bet gene expression in atherosclerotic patients. Methods: Thirty-one patients and 15 healthy controls participated in this study. Healthy control and patients in Vitamin A group received 25 000 IU retinyl palmitate daily for 4 months. Control patients also received 1 pearl of placebo per day up to 4 months. Gene expression levels were assessed by real-time PCR using SYBR green detection method. Results: IFN-γ gene expression in fresh cells of patients taking vitamin A declined slightly (0.85-fold, p = 0.068), whereas the expression of this gene was increased in patients taking placebo, and in healthy control subjects 1.2-fold (p = 0.267) and 1.7-fold (p = 0.580), respectively. There were no significant difference (p = 0.159) between 3 groups in terms of IFN-γ gene expression in cells stimulated with PHA. In order to determine whether PHA stimulation of PBMCs in vitro had an effect on T-bet expression, we measured the difference between the 3 groups of studied. The results showed significant differences between the groups (p = 0.046). IFN-γ gene expression in cells activated with ox-LDL in healthy control subjects and patients taking vitamin A, was reduced 0.43 (p = 0.0001) and 0.41 (p = 0.001) respectively, but in placebo patients was increased 2.2-fold (p = 0.959). Conclusion: Considering role of vitamin A on suppression of Th1 cells in atherosclerotic patients, it can be concluded that vitamin A supplementation may be advantageous for these patients.
    Immunological investigations 12/2014; 44(2). DOI:10.3109/08820139.2014.953635 · 1.90 Impact Factor
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    • "Atherosclerosis (AS) is a chronic inflammatory disease that involves various immune cells, particularly T lymphocytes, such as CD4 + T-helper cells [1] [2]. Acute cerebral Infarction (ACI) is a leading cause of death and the most frequent cause of permanent disability worldwide . "
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    ABSTRACT: CD4(+)CD25(+) regulatory T (Treg) cells and Th17 cells play important roles in peripheral immunity. Oxidized low-density lipoprotein (ox-LDL) is an instrumental factor in atherogenesis. However, the changes of Th17/Treg cells in patients with acute cerebral Infarction (ACI) and impact on Th17/Treg by ox-LDL are not clear. Here, we examined the balance of Th17/Treg in ACI patients and the effect of ox-LDL on this balance. The frequencies of Th17 and Treg cells, key transcription factors and relevant cytokines were examined in patients with ACI, Transient ischemic attack (TIA) and controls. The correlations of cytokines, inflammatory biomarkers and ox-LDL in serum to Th17/Treg frequency, and the effects of ox-LDL on Th17/Treg cells in vitro were analyzed. ACI patients have shown a significant increase of Th17 frequency, RORγt expression and Th17 related cytokines (IL-17 and IL-6 ) levels, and a clear decline of Treg frequency, Foxp3 expression, suppressive function and regulatory cytokines (IL-10 and TGF-β1) levels. Furthermore, TIA patients also have notable variation as compared to control group. Serum ox-LDL and inflammatory biomarkers were positively correlated with the frequency of Th17 cells and negatively correlated with the frequency of Treg cells. Treg and Th17 cells from ACI patients were significantly susceptible to ox-LDL-mediated alterations in vitro. Th17/Treg cells were imbalanced in ACI patients, and ox-LDL may contribute to this imbalance and lead to the occurrence of ACI suggesting their pathogenetic role in ACI.
    International journal of clinical and experimental pathology 01/2013; 6(6):1015-27. · 1.78 Impact Factor
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    • "It is likely that these autoimmune responses initially have a beneficial effect facilitating the removal of potentially harmful antigens [7]. However, studies performed on hypercholesterolaemic mice deficient in different components of innate and adaptive immunity uniformly indicate that the net effect of immune activation is proatherogenic [7] [88] and that atherosclerosis, at least to some extent, should be regarded as an autoimmune disease. "
    The Scientific World Journal 11/2012; DOI:10.1100/TSWJ · 1.73 Impact Factor
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