Article

Expression of inorganic phosphate/vesicular glutamate transporters (BNPI/VGLUT1 and DNPI/VGLUT2) in the cerebellum and precerebellar nuclei of the rat.

Laboratory of Neuroendocrinology, Institute of Basic Medical Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki 305-8575, Japan.
Molecular Brain Research (impact factor: 2). 11/2002; 107(1):23-31. DOI:10.1016/S0169-328X(02)00442-4 pp.23-31
Source: PubMed

ABSTRACT Expression of inorganic phosphate/vesicular glutamate transporters (BNPI/VGLUT1 and DNPI/VGLUT2) was studied in the cerebellum and precerebellar nuclei of rats using immunohistochemistry and in situ hybridization. DNPI/VGLUT2-stained mossy fibers were principally seen in the vermis (lobules I and VIII-X) and flocculus, whereas BNPI/VGLUT1-stained mossy fibers were localized throughout the cortex. Some vermal and floccular mossy fibers were stained for both transporters. High levels of DNPI/VGLUT2 mRNA hybridization signals were demonstrated in many neurons throughout the vestibular nuclear complex as well as the lateral reticular, external cuneate, inferior olivary and deep cerebellar nuclei. Significant BNPI/VGLUT1 mRNA signals were demonstrated in the lateral reticular nucleus and vestibular nuclear complex but not in the inferior olivary nucleus, indicating that climbing fibers have DNPI/VGLUT2 only. These results show that DNPI/VGLUT2 is expressed preferentially to vestibulo-, reticulo- and cuneocerebellar neurons, some of which also possess BNPI/VGLUT1, suggesting some differential and co-operative functions between DNPI/VGLUT2 and BNPI/VGLUT1 in the cerebellum.

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Keywords

BNPI/VGLUT1-stained mossy fibers
 
cerebellar nuclei
 
climbing fibers
 
cortex
 
DNPI/VGLUT2
 
DNPI/VGLUT2 mRNA hybridization signals
 
DNPI/VGLUT2-stained mossy fibers
 
external cuneate
 
floccular mossy fibers
 
inferior olivary
 
inferior olivary nucleus
 
inorganic phosphate/vesicular glutamate transporters
 
lobules
 
possess BNPI/VGLUT1
 
precerebellar nuclei
 
reticulo-
 
Significant BNPI/VGLUT1 mRNA signals
 
situ hybridization
 
transporters
 
vestibular nuclear complex